Cytochrome P450 reductase fusion proteins with

Figure 13.2. A proposal for a simplified classification of CYPs with reference to either use of a ferredoxin or alternative electron transport mechanism. Class la— typical bacterial system supported by ferredoxin and ferredoxin reductase, for example, CYPlOl Class Ib—Rhodococcus sp. CYP fusion protein containing a ferredoxin domain Class Ic—Methylococcus capsulatus CYPS 1-ferredoxin fusion . Class 11a— typical eukaryotic CYP/NADPH-cytochrome P450 reductase system Class lib—a fusion protein of a CYP and flavoprotein reductase, for example, P450gM 3 Class IIc—P450 j containing separate flavodoxin and flavodoxin reductase partners . Class fll—standalone functional CYPs, for example, P450jj. ...

Hotze M, Schroder G, Schrodo- J (1995) Cinnamate 4-hydroxylase fiom Catharanthusroseus, and a strategy for the functional expression of plant cytochrome P450 proteins as translational fusions with P450 reductase in Escherichia coli. FEES Lett 374 345-350... 

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