Oxynitrilases recombinant

In these synthesis, the optically active (R)-cyanohydrin is transformed into the corresponding a-hydroxy carboxylic ester and the hydroxyl funchon is achvated by sulfonylahon. The treatment of the corresponding intermediate with tetra-hydrothieno[3,2-c]pyridine stereoselectively yields the (S)-configured clopidogrel (Scheme 10.23). In the second case, a mutant of the recombinant almond (Pmnus amigdalus) (R)-oxynitrilase isoenzyme 5 catalyzes the formation of enantiopure (R)-2-hydroxy-4-phenylbutyronitrile [54]. Reaction of the sulfonylated hydroxyester derivative with the corresponding dipeptide leads to the formation of enalapril or lisinopril (Scheme 10.24). 

Human interferon-o2a, interleukin-2 receptor, human IgG, human serum albumin, dehydrogenases glucose-6-phosphate dehydrogenase, foanate dehydrogenase, pyruvate decarboxylase, S-oxynitrilase, bovine liver catalase, recombinant protein G, plasmid DMA, human blood coagulation factor VIII, enantiomers of kynurenine, tryptophan, beta-blockers, practolol, thic ntal... 

DSM developed a recombinant oxynitrilase from the rubber tree (Hevea brasiliensis), with which it is possible to produce the cyanohydrin of (S)-3-phenoxy-benzaldehyde with an enantiomeric excess of 98.5 % and a space/time-yield of 1,000 g/l/day. [113]... 

Sosedov, O., Matzer, K., Burger, S., Kiziak, C., Baum, S., Altenbuchner, J., Chmura, A., van Rantwijk, F., and Stolz, A. (2009) Construction of recombinant Escherichia coli catalysts which simultaneously express an (S)-oxynitrilase and different nitrilase variants for the synthesis of (S)-mandelic acid and (S)-mandelic amide from benzaldehyde and cyanide. Adv. Synth. Catal., 351, 1531-1538. 

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