Rubredoxin oxygen oxidoreductase

Chen L, Liu M-Y, LeGall J, et al. 1993b. Purification and characterization of an NADH-rubredoxin oxidoreductase involved in the utilization of oxygen by Desulfovibrio gigas. J Biochem 216 443-8. 

Complex heme proteins which contain redox centers other than just electron transfer hemes are normally associated with enzymatic catalysis. In this section, we will refer to some of the best studied complex heme proteins sulfite reductase, nitrite reductase, formate dehydrogenase, fumarate reductase and rubredoxin-oxygen oxidoreductase. 

A new member of the family of nonheme diiron enzymes recently discovered is called rubrerythrin. This metalloprotein is formally classified as an oxidoreductase (rubredoxin oxygen oxidoreductase). The diiron(III,III) active site structure is displayed in Figure 2(f). This biomolecule possesses two histidines coordinated to one iron and one histidine coordinated to the second iron. A carboxylate bridges the two irons and there are carboxylate ligands also coordinated to each iron. The purpose of this enzyme in the strict anaerobe is to safely reduce oxygen to water. 

ACP = acyl carrier protein ACPA D = ACPA desat-urase AlkB = octane 1-monooxygenase AOX = alternative oxidase DMQ hydroxylase = 5-demethoxyquinone hydroxylase EXAFS = extended X-ray absorption fine structure spectroscopy FMN = flavin mononucleotide FprA = flavoprotein A (flavo-diiron enzyme homologue) Hr = hemerythrin MCD = magnetic circular dichroism MME hydroxylase = Mg-protophorphyrin IX monomethyl ester hydroxylase MMO = methane monooxygenase MMOH = hydroxylase component of MMO NADH = reduced nicotinamide adenine dinucleotide PAPs = purple acid phosphatases PCET = proton-coupled electron transfer, PTOX = plastid terminal oxidase R2 = ribonucleotide reductase R2 subunit Rbr = rubrerythrin RFQ = rapid freeze-quench RNR = ribonucleotide reductase ROO = rubredoxin oxygen oxidoreductase XylM = xylene monooxygenase. 

RUBREDOXIN-OXYGEN OXIDOREDUCTASE AND NITRIC OXIDE REDUCTASES... 

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