Overpressured layer chromatography applications

L. Botz, Sz. Nyiredy and O. Sticher, Applicability of long distance overpressured layer chromatography , J. Planar Chromatogr. 4 115-122 (1991). 

Sz. Nyiredy, Application of the PRISMA" Model for the Selection of Eluent-systems in Overpressure Layer Chromatography (OPLC), Labor MIM, Budapest, 1987. 

The second example relates to the separation and determination of steroid isomers. The separation of R- and S-budesonide, syn- and antinorgestimate, -norgestreloxime, and 6-hydroxy-norgesti-mate have been done on silica, and amino-bonded silica stationary phases by conventional and overpressurized layer chromatography (OPLC) (35). Application of amino-bonded silica as stationary phase, and chloroform-diethyl ether-methyl ethyl-ketone (4 3 3, v/v) as mobile phase results in a satisfactory separation of the isomer pairs, especially, if OPLC technique is used. 

At present attempts are being made to optimize the chromatography, and a great deal of faith has been placed in OPTLC (overpressured thin-layer chromatography). With this technique an extension of the development distance, better resolution and a shorter separation time are achieved. In 1982 a report was published on the applicability of this technique in the analysis of various substance groups 59). 

T3dhak, E. Mincsovics, E. Teten), P. Zamh6,1. Kalasz, H. Applicability of overpressured thin-layer chromatography in ph3ftochemistry. Acta Horticult. 1980, 96,113. 

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