Other specific biochemical markers for Purkinje cells

Other specific biochemical markers for Purkinje cells 

Several polypeptides, that are present in all Purkinje cells, but not in other cells of the cerebellum, have been mentioned in the previous sections of this chapter. They include the IP3 receptor (identical to the P400 protein and to the PCPP-260 protein of Walaas et al., 1986) (see Section 3.1.4), IPj-S-kinase (Section 3.1.4), cGMP-dependent protein kinase (Section 3.1.5), PEP-19 and calbindin-D28K (Section 3.1.7). Two other 

Purkinje cell-specific markers include several glyco- and phosphoproteins, peptides, antigenic determinants that have not been identified or determinants that Purkinje cells share with other, non-cerebellar cell types. One of the first sera specific for rat Purkinje cells was obtained, using immunohistochemical screening, by Woodhams et al. (1979), but the antigen corresponding to this antibody has not been identified. Reeber et al. 

L-7 is a protein specific for Purkinje cells. Labelling with polyclonal antibodies against predicted L-7 sequences was present in somata, including the nucleus, in dendrites and dendritic spines, and in axon and axon terminals of Purkinje cells. All Purkinje cells, but no other types of cerebellar neurons appeared to be labelled (Berrebi and Mugnaini, 

The expression of the L-7 gene by all adult Purkinje cells of the rat cerebellum was reported by Nordquist et al. (1988, their PCD5 clone), Oberdick et al. (1990) Vandaele et al. (1991, their Purkinje cell protein-2) and Smeyne et al. (1991). According to Oberdick et al. (1990) and Berrebi et al. (1991) the L-7 gene is also expressed by retinal 

---