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Other DNA Amplification Techniques

There are other techniques that can be used to amplify DNA. For instance, the ligase chain reaction (LCR) was achieved on a Si-glass chip for detecting known [Pg.310]

In another report, the DNA amplification was based on strand displacement amplification (SDA). This method worked at an isothermal temperature of 50°C for the 106-bp product from Mycobacterium tuberculosis [942]. [Pg.311]

Another method is loop-mediated isothermal DNA amplification (LAMP). This was carried out at a constant temperature of 65°C on the sample well of a PMMA chip. Subsequent CGE separation was carried out. The LAMP method has been applied for analysis of the PSA gene with a 23-fg/p.L template concentration [953,954], [Pg.311]


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Amplification techniques

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