OSPED

CgHi OsP t295 C3H3404S14 o21 CjHioNj a296, pl21... 

Results of quantum-mechanical calculations (Dunitz and Orgel, 1957) have given values for the OSPEs of a number of transitional metal ions and the degree of inversion of mixed spinels... 

In principle, on-line SPE-LC can be automated quite easily as well, for instance, by using Such programmable on-line SPE instrumentation as the Prospekt (Spark Holland) or the OSP-2 (Merck) which have the capability to switch to a fresh disposable pre-column for every sample. Several relevant applications in the biomedical field have been described in which these devices have been used. Eor example, a fully automated system comprising an autosampler, a Prospekt and an LC with a UV... 

F. 6 Schematic representation of HPLC-HPTLC coupling by means of the OSP-2 system (Merck) for post-column enrichment of the column eluate fractions. 

Figure 5. The oocyte c-mos promoter. Efficient transcription in oocytes requires only a minimal promoter fragment, extending from -21 to +20. This fragment includes an Inr-like element, which is required for efficient gene expression. A potential binding site for the oocyte-specific transcription factor OSP-1 is located further upstream.

An additional sequence element within the mouse ZP3 promoter has been identified as the binding site of an oocyte-specific protein called OSP-1 (Schickler et al., 1992), although the possible effect of mutations of this element on transcriptional activity has not been established. Nonetheless, it is interesting that a similar sequence (GATGA) is present 40 nucleotides upstream of the c-mos transcription initiation site (Fig. 5), although deletion of this element had no discernible effect on the activity of the c-mos promoter in microinjected oocytes (Pal et al., 1991). 

Department of Health and Human Services, National Institutes of Health. "Disease-specific Estimates of Direct and Indirect Costs of Illness and NIH Support." (2000). wwwl.od.nih.gov/osp/ospp/ecostudies/COIreportweb.htm... 

Commercially available fully automated online SPE systems include Prospekt, Prospekt-2, and Symbiosis systems (Spark Holland, the Netherlands) and Merck s OSP-2 (Darmstadt). Figure 10.2 shows a Symbiosis (Kuklenyik et al. 2005) two-cartridge, single analytical column online SPE... 

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