Organophosphorus pesticides coumaphos

Organophosphorus pesticides, e.g. coumaphos, menazon, maretin, dursban 200 °C, 45 min Induced fluorescence or amplification of natural fluorescence, detection limits 1-80 ng. [21]... 

Organophosphorus pesticides, e.g. coumaphos, menazon, maretin, dursban... 

Brown RL, Farmer CN, Millar RG. 1987. Optimization of sweep codistillation apparatus for determination of coumaphos and other organophosphorus pesticide residues in animal fat. J Assoc Off Anal Chem 70(3) 442-445. 

Another organophosphorus insecticide, Bayrusil (XIII) becomes fluorescent upon heat treatment but the fluorescence yield is less than with coumaphos (around 0.01 yg/spot) (4 4). This pesticide has also been analysed in foodstuffs at the 0.02 ppm level (46 ). ... 

Organophosphorus hydrolase (OPH, EC 3.1.8.1) is a homodimer with a binuclear metal center. OPH has broad substrate specificity and can hydrolyze organophosphate pesticides such as methyl paradiion, ediyl parathion, paraoxon, chlorpyrifos, coumaphos, cyanophos and diazinoa Table I 9,12-14). The enzymatic hydrolysis rates are 40 - 2450 times faster than chemical hydrolysis at pH 7.0 and the enzyme is reported to be stable at ten ratures of up to 4S-S0°C (3). However, hydrolysis rates varied from very fast for phosphotriesters and phosphothiolester pesticides (P-0 bond) such as paraoxon (ken > 3800s ) and coumaphos (kcat = 800s ) to limited hydrolysis for Diazinon (kcat == 176 s ) and fensulfothion (l a, = 67 s ) (14). 

Organophosphorus hydrolase (OPH), a biological catalyst, was shown to effectively hydrolyze a range of organophosphate esters pesticides such as parathion, coumaphos, and acephate and chemical warfare agents such as soman, satin, VX, and tabun. " The enzyme was first discovered in soil microorganisms, i.e.. Pseudomonas diminuta and Flavobacterium spp. ... 

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