Organic Phase Size-exclusion

The average molecular mass of synthesized polymers can be measured by SEC. This system has been called gel-permeation liquid chromatography, using porous polystyrene gel as the stationary phase material. Polymers are usually dissolved in THF or chloroform for separation by SEC. The molecular density 

Hirukawa, and T. Hanai, Nihon Kagaku Kaishi, 1986,969. 

Schill, R. Modin, and B.-A. Person, Acta Pharm. Seuc., 1965,2, 119. 

Lafosse, P. Agbo-Hazoume, B. Chaabane-Doumandji, M. Gibert, and Y. Levi, J. Chromatogr., 1986,354, 116. 

in Thin-layer chromatography , ed. G.B. Marini-Bettola, Elsevier, Amsterdam, 1964, p. 75. 

---

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Principles and Instrumentation. Atomic Fluorescence Spectrometry. Gas Chromatography Pyrolysis Mass Spectrometry. Liquid Chromatography Normal Phase Reversed Phase Size-Exclusion. Polarography Inorganic Applications Organic Applications. Polymers Synthetic. Thin-Layer Chromatography Overview. Voltammetry Organic Compounds. 

Microwave-Assisted Solvent Extraction Pressurized Fluid Extraction Supercritical Fluid Extraction Solid-Phase Extraction Solid-Phase Microextraction. Gas Chromatography Overview, Mass Spectrometry Environmental Applications. Immunoassays Overview. Liquid Chromatography Overview Reversed Phase Size-Exclusion Liquid Chromatography-Mass Spectrometry. Pesticides. Supercritical Fluid Chromatography Overview Applications. Thin-Layer Chromatography Overview. Water Analysis Organic Compounds. 

For the size exclusion chromatography of proteins on silica-hased diol packings, it is generally recommended to use fully aqueous mobile phases with a salt concentration between 0.1 and 0.3 M. In general, a phosphate buffer around pH 7 is used as the mobile phase. Under these circumstances, the tertiary structure of most proteins is preserved without difficulty and the interaction of proteins with each other is minimized. However, other inorganic buffers or combinations of buffers with organic solvents can be used without difficulties for special applications. 

Exclusion chromatography is a technique for separating molecules based on their effective shape and bulkiness. The method is generally called size-exclusion chromatography (SEC). When the mobile phase is an organic solvent or a mixture of organic solvent, the technique is called gel permeation chromatography (GPC), in the case of aqueous mobile... 

Surface-modified silica gels are used for a variety of separations, but organic polymer-based stationary phase materials are more useful for long-term operations, such as for an amino acid analyser and size-exclusion liquid chromatography. 

---