Opiates quantification

Scheidweiler KB, Huestis MA. 2004. Simultaneous quantification of opiates, cocaine, and metabolites in hair by LC-APCI-MS/MS. Anal Chem 76 4358. 

Sadzot, B., Mayberg, H.S., Frost, JJ. Detection and quantification of opiate receptors in man by positron emission tomography. Potential applications to the study of pain. Neurophysiol. Clin. 20(5), 323-334, 1990. 

Jurado, C., Gimenez, M. R, Menendez, M., Repetto, M., Simultaneous Quantification of Opiates, Cocaine and Catmabinoids in Hair, Forensic Sci. Int., 70,165,1995. 

The characterisation and quantification of opiates (morphine, 6-MAM, codeine, and ethylmorphine) are obtained with the aid of a GC/MS. The apparatus and the conditions used are the following ... 

In these conditions, the detection limits are 30 pg/mg for codeine, ethylmorphine, and morphine, and 50 ppb for 6-MAM, while the quantification limits are 100 pg/mg for the first three opiates and 200 pg/mg for 6-MAM. These values are comparable to those obtained by Moeller et al., who proposed LODs of 100 pg/mg for opiates. 

We have demonstrated in particular that SFE is an appropriate method for analysing opiates in hair. It is rapid (30 min), efficient, and reproducible. The procedure applied to standard hair, or to hair coming from drug abusers, has given excellent results. In addition, the quantification limits are comparable to those generally observed in hair analysis. 

Like head hair, axillary or pubic hair can be easily tested with gas chromatography/mass spectrometry (GC/MS) for detection and quantification of the opiates after decontamination by washing and acidic hydrolysis according to previous... 

When quantifying opiates by HPLC, a number of basic principles should first be considered before the quantification process is undertaken. 

Cordero R, Paterson S (2007) Simultaneous quantification of opiates, amphetamines, cocaine and metabolites and diazepam and metabolite in a single hair sample using GC-MS. J Chromatogr B 850 423—431... 

Mason KE, Partridge EL, Stockham PC, Felgate PD. Quantification of opiates on postmortem samples using HPLC ESI-QTOF-MS and MS/MS, Poster presentation. 20th International Symposium on the Forensic Sciences, 5—9 September 2010, Sydney, Australia. 

Miller El, Wylie FM, Oliver JS. Simultaneous detection and quantification of amphetamines, diazepam and its metabolites, cocaine and its metabolites, and opiates in hair by LC-ESI-MS-MS using a single extraction method. J Anal Toxicol 2008 32(7) 457-69. 

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