Open reading frame entries

Molecular evidence that a functionally unassigned open reading frame in Arabidopsis does, in fact, encode a putative MIOase has just been reported (Lorence et al., 2004). In this study, MIOase provides a possible entry point into AsA biosynthesis. Those concerned with MIOase and its role in the MIOP will now have the opportunity to extend these findings to breakdown products of AsA catabolism (Banhegyi and Loewus, 2004) as well as to cell wall biogenesis. 

Figure 5.5. The generic structure of a eukaryotic niRNA. illustrating some post-transcriptional regulatory elements that affect gene expression. Abbreviations (from 5 to 3 ) UTR. untranslated region inVG, 7-inelhyl-guanosinc cap Hairpin, hairpin-like secondary structures uORF, upstream open reading frame IRES, internal ribosome entry site CPE. cytoplasmic poiyadenylation element AAUAAA. polyadenyla-...

The hard link concept is much easier conceptually than neighboring. Hard links are applied between entries in different databases and exist everywhere there is a logical connection between entries. For instance, if a PubMed entry talks about the sequencing of a cosmid, a hard link is established between the PubMed entry and the corresponding nucleotide entry. If an open reading frame in that cosmid codes for a known protein, a hard link is established between the nucleotide entry and the protein entry. If, by sheer luck, the protein entry has an experimentally deduced structure, a hard link would be placed between the protein entry and the structural entry. The hard link relationships between databases is illustrated in Figine 7.1. 

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