Online continuous coating

The study aims at the development of a production line for readily manufactured reactors with integrated catalysts as marketable products. The archetype for such an online technology was found in continuous coating and mass-produce processes in the polymer industry for the production of non-woven textiles and foils. A typical scheme for the melt-coating of a polymer foil is shown in Figure 4.106 [165],... 

Acetylcholineesterase and choline oxidase A glassy C electrode surface was modified with osmium poly (vinyl-pyridine) redox polymer containing horseradish peroxidase (Os-gel-HRP) and then coated with a co-immobilized layer of AChE and ChO. A 22 pL pre-reactor, in which ChO and catalase were immobilized on beads in series, was used to remove choline. The variation in extracellular concentration of ACh released from rat hippocampal tissue culture by electrical stimulation was observed continuously with the online biosensor combined with a microcapillary sampling probe. Measurement of ACh and Ch was carried out by using a split disc C film dual electrode. 

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