On-Line HPLC Detection

With such an optimized system, analytical runs within 20-60 sec are possible. From the by-pass of the main stream, samples are automatically injected in time intervals of At (e.g., between 20 and 60sec). Once the impurity (Imp.) to be removed has reached its critical value, fractioning into another vessel takes place. Ideally, the outlet tube between sampling point and fraction vessel is adjustable and designed in such a way that the analytical result is 

Further progress can be expected since new column materials (e.g., monolithic columns) and further improved FIPLC systems (e.g., ultra performance systems) have been developed to speed up analytical runs significantly. Their use as routine on-line detection tools will improve the productivity of preparative FIPLC not only in applications where broad overloaded peaks are observed, but also in cases where high concentrations of different solutes elute close to each other, an inherent aspect of displacement chromatography systems for example. 

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CARDENOSA R, MOHAMED R, PINEDA M and AGUILAR M (2002) On-line HPLC detection of tocopherols and other antioxidants through the formation of a phosphomolybdemun complex, /dgricihod Chem, 50, 3390-5. 

Tire three strong Tell lines were used as sensitive emission lines for the determination of Tc by inductively coupled plasma optical emission spectrometry (ICP-OES), which achieves a detection limit of around 4 pg Tc/1. The convenience of this approach makes it particularly suitable for on-line HPLC detection in speciation studies at enhanced environmental technetium levels [39]. Examples of this type of procedure for the determination of elements arc described in reviews [40,41 ]. 

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