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Oligosaccharide structural analysis

The CFR 21 part 11 compliant software newly released by Convergent Bioscience makes imaged cIEF a viable technique for use in QC environments for release testing of therapeutic proteins or antibody molecules. i-cIEF has also been used heavily for characterization of protein modifications such as deamidation and isomerization, as well as oligosaccharide structure analysis. ... [Pg.378]

Recent Developments in the Use of 13C-N.m.r. Spectroscopy for the Structural Analysis of Oligosaccharides of Glycoproteins... [Pg.8]

Dell, A., Morris, H.R., Easton, R., Haslam, S.M., Panico, M., Sutton-Smith, M., Reason, A.J. and Khoo, K.-H. (1999b) Structural analysis of oligosaccharides FAB-MS, ES-MS and MALDI-MS. In Ernst, B., Sinay, P. and Hart, G. (eds) Oligosaccharides in Chemistry and Biology - a Comprehensive Handbook. Wiley-VCH, Weinheim (in press). [Pg.310]

Due to extensive overlap within the region 5 3.0-4.5 ppm, H NMR spectra of oligosaccharides in many cases produce complex patterns. In one-dimensional NMR analysis, the solvent pyridine-ds improves signal dispersion better than methanol- /4 or acetone-dg. For structural analysis, the following steps are suggested ... [Pg.126]

Nymphaea caerulea, for seven natural anthocyanins stabilizing a DNA triplex, etc. Sequential analysis of the oligosaccharide structures of the flavonol tamarixetin-7-O-rutino-side has been performed by ID multistep-relayed COSY-ROESY experiments. Selective excitation was performed by Gaussian-shaped soft pulses. [Pg.48]

Schroeder, H. and Haslinger, E., Sequential analysis of oligosaccharide structures by selective NMR techniques. Part II. ID multistep-relayed C0SY-R0ESY, Liebigs Ann. Chem., 1, 751, 1993. [Pg.123]

Fig. 4.5.8 Examples for structures and molecular masses of 2-aminobenzamide (2-AB)-labelled oligosaccharide moieties derived from serum transferrin. Values below the oligosaccharide structures indicate the expected masses (in Da) by matrix-assisted laser desorption ionisation - time of flight analysis. Fig. 4.5.8 Examples for structures and molecular masses of 2-aminobenzamide (2-AB)-labelled oligosaccharide moieties derived from serum transferrin. Values below the oligosaccharide structures indicate the expected masses (in Da) by matrix-assisted laser desorption ionisation - time of flight analysis.
CH3C02CH0 would affotd fragment 79. This structural analysis demonstrates the potential of the technique for structural studies of oligosaccharides. [Pg.261]

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See also in sourсe #XX -- [ Pg.323 ]

See also in sourсe #XX -- [ Pg.915 ]



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