Oligosaccharides enzymatic

F. Ouame and A. Guibert, Fmcto-oligosaccharides Enzymatic synthesis from sucrose, Zuckerind, 120 (1995) 793-798. 

S. Kojima, T. Hasegawa, T. Yonemura, K. Sasaki, K. Yamamoto, Y. Makimura, T. Takahashi, T. Suzuki, Y. Susuki, and K. Kobayashi, Ruthenium complexes carrying a disialo complex-type oligosaccharide Enzymatic synthesis and its application to a luminescent probe to detect influenza viruses, Chem. Commun. (2003) 1250-1251. 

Proteinase Inhibitor Inducing Factor Activity in Tomato Leaves Residues in Oligosaccharides Enzymatically Released From Cell Walls. Proceedings National Academy of Science DSA 78, 3536-... 

Ouame F, Guibert A (1995) Fructo-oligosaccharides enzymatic synthesis from sucrose. Zuckerindustrie 120 793-798... 

Enzymatic Synthesis of Oligosaccharides Enzymatic Synthesis of Polysaccharides Enzymatic Synthesis of Glycoproteins Enzymatic Synthesis of Glycolipids Glycosylation of Natural Products... 

W4. Weissmann, B., Meyer, K., Sampson, P., and Linker, A., Isolation of oligosaccharides enzymatically produced from hyaluronic acid. J. Biol. Chem. 208, 417-429 (1954). 

In sycamore maple Acer pseudoplatanus) and tomato 118 Lycopersicon esculentum) cell Activity resides in oligosaccharides enzymatically released... 

Bishop, P.D., Makus, D.J., Pearce, G., and Ryan, C.A. (1981). Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymatically released from cell walls. Proc. Natl Acad. Sci. USA 78, 3536-3540. 

Figure 10.32 Applications of bidirectional chain extension for the synthesis of disaccharide mimetics and of annulated and spirocyclic oligosaccharide mimetics using tandem enzymatic aldol additions, including racemate resolution under thermodynamic control.

To allow all culture productiou to be coutrolled, a method for rapid analysis is required. Prior to development of an LC-MS method, the analysis was both complex and time-consuming, involving the purification of a relatively large amount of the antibody using affinity chromatography, enzymatic release, and subsequent derivatizafion of the oligosaccharides and their analysis by using capillary electrophoresis. 

Cyclodextrins (CDs) are inclusion compounds formed by enzymatic decomposition of starch to the cyclic oligosaccharides containing six to eight... 

Eujii, M., Rapid enzymatic transclyco-sflation and oligosaccharide synthesis in a microchip reactor. Lab. Chip 2 (2002)... 

Nishio, T., Hakamata, W., Ogawa, M. et al. (2005) Investigations of a useful a-glycosidase for the enzymatic synthesis of rare sugar oligosaccharides. Journal of Applied Glycoscience, 52, 153-160. 

Murata, T. and Usui, T. (2006) Enzymatic synthesis of oligosaccharides and neoglycoconjugates. Bioscience, Biotechnology, and Biochemistry, 70, 1049-1059. 

Rabbani, S., Schwardt, O. and Ernst, B. (2006) Glycosyltransferases an efficient tool for the enzymatic synthesis of oligosaccharides and derivatives as well as mimetics thereof. Chimia, 60, 23-27. 

Several enzymatic routes with sucrase-type enzymes have been established for oligosaccharide synthesis, some of which are applied industrially for food and feed ingredients, and important examples are compiled.5 Convenient routes for new oligosaccharides, however, are rarely available. 

This report surveys new routes for the synthesis of oligosaccharides, with emphasis on enzymatic reactions, since they offer unique properties, proceeding highly regio-and stereo-selectively in water solution, and generally afford high yields. Summarized... 

Fig. 8. Different products with sucrose analogues as substrates.115 Enzymatic synthesis of 1-kestose, 1-nystose, and their analogues by /(-fructofuranosidase of A. niger. Structures of fructo-oligosaccharides (A) commercial products, (B) mannose- (C) galactose-, and (D) xylose-substituted analogues.

T. Murata and T. Usui, Enzymatic synthesis of oligosaccharides and neoglyco-conjugates, Biosci. Biotechnol. Biochem., 70 (2006) 1049-1059. 

Incomplete (N-linked) glycosylation prompts decreased in vivo activity due to more rapid hepatic clearance of the EPO molecule. Enzymatic removal of terminal sialic acid sugar residues from oligosaccharides exposes otherwise hidden galactose residues. These residues are then free to bind specific hepatic lectins, which promote EPO removal from the plasma. The reported plasma tm value for native EPO is 4-6 h. The tm for desialated EPO is 2 min. Comparison of native human EPO with its recombinant form produced in CHO cells reveals very similar glycosylation patterns. 

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