Enzymatic Synthesis of Oligosaccharides

4 Enzymatic Synthesis of Oligosaccharides and Glycoconjugates on Soluble Supports 

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Murata, T. and Usui, T. (2006) Enzymatic synthesis of oligosaccharides and neoglycoconjugates. Bioscience, Biotechnology, and Biochemistry, 70, 1049-1059. 

Rabbani, S., Schwardt, O. and Ernst, B. (2006) Glycosyltransferases an efficient tool for the enzymatic synthesis of oligosaccharides and derivatives as well as mimetics thereof. Chimia, 60, 23-27. 

T. Murata and T. Usui, Enzymatic synthesis of oligosaccharides and neoglyco-conjugates, Biosci. Biotechnol. Biochem., 70 (2006) 1049-1059. 

The ability of the hyperthermophilic glycosynthases to promote the synthesis of oligosaccharides is a clear example of how the unique characteristics of stability to high temperatures and acidic pH of the glycosidases from hyperthermophilic archaea allows the development of a novel strategy for the chemo-enzymatic synthesis of oligosaccharides. 

Moracci, M., Trincone, A., Cobucci-Ponzano, B., Perugino, G., Ciaramella, M., and Rossi, M. (2001) Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases of Sulfolobus solfataricus. Extremophiles. 5 145-152. 

Chen, X., Kowal P., and Wang, P.G. Large-scale enzymatic synthesis of oligosaccharides, Curr. Op. Drug Disc. Devel., 3 756-763 (2000). 

Enzymatic Synthesis of Oligosaccharides Enzymatic Synthesis of Polysaccharides Enzymatic Synthesis of Glycoproteins Enzymatic Synthesis of Glycolipids Glycosylation of Natural Products... 

Sujino K, Uchiyama T, Hindsgaul O, Seto NOL, Wakarchuk WW, Palcic MM. Enzymatic synthesis of oligosaccharide analogues UDP-Gal analogues as donors for three retaining a-galactosyltransferases. J. Am. Chem. Soc. 2000 122 1261-1269. 92. 

Chemoenzymatic techniques provide relatively fast routes to complex oligosaccharide structures. Nevertheless, they always combine biochemical and synthetic elements and therefore require the cooperation of both fields. To date, few glycosyltransferases have been cloned, expressed, and produced in quantities sufficient for preparative enzymatic syntheses. Given the advantages of enzymatic synthesis of oligosaccharides over traditional schemes, research into overexpression of glycosyltransferases will continue to flourish and will offer the benefit of preparative enzymology techniques for carbohydrate chemists in the future. 

Enzymatic synthesis of oligosaccharides with (J-l,4-galactosyl-transferase and [Pg.220]

Nishimura and coworkers 29f> 297 described a novel method for the enzymatic synthesis of oligosaccharide derivatives employing an a-chymotrypsin sensitive linker. The synthesis of the water soluble GlcNAc-polymer 197, sensitive to a-chymotrypsin, is shown in Fig. 18-27. Oxazoline derivative 193 was coupled with 6-(N-benzyloxycarbonyl-L-phenylalanyl)-amino-hexanol-l (194) followed by N-depro-tection of the phenylalanine and subsequent condensation with 6-acrylamido caproic acid 195. De-O-acetylation gave the polymerizable GlcNAc derivative 196. Finally, copolymerization of acrylamide and monomer 196 in the presence of ammoniumper-sulphate (APS) and N,N,N, N -tetramethyl ethylene diamine (TMEDA) gave the... 

Doris Su et al. review the enzymatic synthesis of oligosaccharides and their conversion to glycolipids. 

The use of specific exo- and endoglycosidases for the sequence determination of glycans has been described (subsection 6.2.3). Thus experiments can be designed to test the structural possibilities of complex glycans. Glycotransferases and glycosidases have been employed for enzymatic synthesis of oligosaccharides and can be used to remodel... 

Ebrahimi M, Placido L, Engel L, Ashaghi KS, and Czermak P. A novel ceramic membrane reactor system for the continuous enzymatic synthesis of oligosaccharides. Desalination 2010 250 1105-1108. 

Enzymatic synthesis of oligosaccharides has long been recognized as a desirable and efficient means of accessing complex carbohydrate structures. This is largely due to the fact that... 

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