Oligosaccharide branching pattern determination

In general, MS/MS allows one to determine the sequence and the branching pattern of oligosaccharides. The isomer position of each of their glycosidic bonds also can be... 

C signals of oligosaccharides related to arabinoxylan. H n.m.r. methods were used to determine the branching patterns in a ceramide pentadecasaccharide differences were noted between the... 

The action patterns of a purified, intracellular dextranase and three intracellular a-D-glucosidases from Pseudomonas UQM733 on pure isomalto-oligosaccharides have been studied. The a-D-glucosidases had optimal activity on isomaltotetraose and were therefore classified as oligoglucanases . They have been used to determine the structure of two branched isomalto-oligosaccharides obtained by enzymic degradation of dextran. 

A GC/MS method, commonly called linkage analysis or methylation analysis, has also proven to be a successful approach to determining interresidue linkage and branch points [14]. It involves complete alkylation of all free hydroxyl groups. The fully methylated oligosaccharides are hydrolyzed into monomers, which are further reduced and acetylated to provide partially methylated alditol acetates (PAMAs). Acetylation takes place at those positions that were newly exposed by hydrolysis and reduction. The GC retention times and fragmentation patterns can reveal the mode of attachment in the native sugar. 

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