Oligopeptides mass spectrometry

Welker, M. Brunke, M. Preussel, K. Lippert, I. von Dohren, H. Diversity and distribution of microcystis (Cyanobacteria) oligopeptide chemotypes from natural communities studied by single-colony mass spectrometry. Microbiology 2004,150, 1785-1796. 

F Lynen, Y Zhao, C Becu, F Borremans, P Sandra. Considerations concerning interaction characterization of oligopeptide mixtures with vancomycin using affinity capillary electrophoresis-electrospray mass spectrometry. Electrophoresis 20 2462-2474, 1999. 

Bowers, W. D., Delbert, S.S., Hunter, R. L. und Mclver. R. T., Jr., Fragmentation of Oligopeptide Ions Using Ultraviolet Laser Radiation and Fourier Transform Mass Spectrometry. J. Am. Chem. Sac. 1984,106, 7288-7289. 

Fastner, J., Erhard, M., and von Ddhren, H., Determination of oligopeptide diversity within a natural population of Microcystis spp. (Cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of flight mass spectrometry, Appl. Environ. Microbiol, 67, 5069, 2001. 

Abstract Hyphenation of electrospray ionization witii ion-trap multistage mass spectrometry (ESI-MS°) provides charactoisation of subtle molecular structure of both syntiietic and natural aliphatic polyesters as well as oligomeric products of their biodegradation. Implementation of multisti e MS technique for structural studies of the novel bio-inspired polymers including poly[(R,S)-3-hydroxybutyrate], a-PHB, telechelics and a-PHB conjugates with oligopeptides has been demonstrated. The novel results concerned with evaluation of the subtle structure of macroinitiators obtained by partial depolymerisation of selected natural poly(3-hydroxyalkanoate)s, PHA, are also presented. 

M. Barber, R.S. Bordoli, R.D. Sedswick, A.N. Tyler, E.T. Whalley, Fast atom bombardment mass spectrometry of bradykinin and related oligopeptides, Biomed Mass Spectrometry of bradykinin and related ohgopeptides, Biomed. Mass Spectrom. 8 337-340 (198 )... 

D.M. Desiderio, The elucidation of primary structure of oligopeptides of biological importance via mass spectrometry. Proc. of the Second Amer. Peptide Symp. (Gordon and Breach, New York) 1972, pp. 159-168... 

Desiderio DM, Sabbatini JZ. Field desorption collision activation linked scanning mass spectrometry of underivatized oligopeptides. Biol Mass Spectrom. 2005 8 565. 

Mass spectrometry has been suggested for the identification of phenylthio-hydantoins produced during the classical and automated sequencing process and a combined gas-liquid chromatography-mass spectrometry technique for amino-acids from oligopeptide hydrolysates has been discussed. "... 

The fundamental component of the system is again cyanamide, but for optimum yields, the addition of AICA HCl and high-energy phosphate (in this case ATP) is required. The amino acid solution with the other components is heated after evaporation, at temperatures which may vary from 40 to 90°C, for up to 24 hours. Upon separation by thin-layer chromatography the peptides are derivatized and identified by combined gas chromatography-mass spectrometry. After 1 hour reaction at 88°C the yield of L-phenylalanine dipeptide was 36% and that of the L-isoleucine dipeptide 22%. The yield and number of oligopeptides obtained from L-phenylalanine increased with temperature and time, with about 66% of the amino acid being converted to a mixture of di-, tri- and tetrapeptides in 24 hours at 90°C.< >... 

Barber, M., P. Jolles, E. Vilkas, and E. Lederer Determination of Amino acid Sequences in Oligopeptides by Mass Spectrometry. I. The Structure of Fortuitin, an Acylnonapeptide Methyl Ester. Biochem. Biophys. Res. Comm. 18, 469 (1965). 

Das, B.C., S.D. Gero, and E. Lederer Detection and Localization of N-Methyl-aminoacid Residues in N-Acyl oligopeptides Methyl Esters by Mass Spectrometry. Nature 217, 547 (1968). 

Bricas, E., J. Van Heijenoort, M. Barber, W.A. Wolstenholme, B.C. Das, and E. Lederer Determination of Amino Acid Sequences in Oligopeptides by Mass Spectrometry. IV. Synthetic N-Acyl Oligopeptide Methyl Esters. Biochemistry 4,2254 (1965). 

Van Hejenoort, J., S. E. Brica, B. C. Das et E. Lederer Determination de sequence d acides amine dans des oligopeptides par la spectrometrie de masse. IX. (A) Acylation avec de nouveaux radicaux mixtes, (B) Peptides contenant des acides amines trifonctionnels. Tetrahedron 23, 3403 (1967). 

Laneelle, G., J. Asselineau, W.A. Wolstenholme, and E. Lederer Determination de sequences d acides amines dans des oligopeptides par la spectrometrie de masse. III. Structure d un peptidolipide de Mycobacterium johnei. Bull. Soc. Chim. Fr. 2133 (1965). 

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