Oligonucleotide targeting fluorescence

Beaucage S, Bergstrom DE, Click GD, Jones RA, eds. Current Protocols in Nucleic Acid Chemistry. 2000. John Wiley Sons, New York. Silverman AP, Kool ET. Oligonucleotide probes for RNA-targeted fluorescence in situ hybridization. Adv. Clin. Chem. 2007 43 79-115. 

Macnaughton S. J., Booth T., Embley T. M., and O Donnell A. G. (1996) Physical stabilization and confocal microscopy of bacteria on roots using 16S rRNA targeted, fluorescent-labeled oligonucleotide probes. J. Microbiol. Meth. 26(3), 279-285. 

Dedysh S. N., Dunfield P. F., Derakshani M., Stubner S., Heyer J., and Liesack W. (2003) Differential detection of type II methanotrophic bacteria in acidic peatlands using newly developed I6S rRNA-targeted fluorescent oligonucleotide probes. FEMS Microbiol. Ecol. 43, 299-308. 

Baker et al. [95] were the first to report the formation of DNA-SWCNT adducts in solution for DNA hybridization. The DNA-SWCNT complexes were synthesized by reacting oxidized SWCNTs with thionyl chloride and ethylenediamine to form amine-terminated sites, shown in Fig. 14.16. The amines were further reacted with succinimidyl 4-(Af-maleimidomethyl)cyclohexane-l-carboxylate (SMCC) forming maleimide groups which reacted with thiol-modified DNA. To confirm covalent attachment and to test the accessibility of the DNA-modified SWNTs, hybridization studies were conducted using fluorescently labeled DNA oligonucleotide targets. Shortly thereafter, the use of carbodiimide-assisted coupling... 

R. E. Hicks, R. I. Amann, and D. A. Stahl, Dual staining of natural bacterioplankton with 4, 6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences, Appl. Environ. Microbiol. 571 2158 (1992). 

Liebermann T, Knoll W (2003) Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir 9 1567-1572... 

Oligonucleotide probes may be labeled with small fluorescent molecules for detection of hybridization by luminescence. Fluorescent probes are widely used in assay systems involving bio-specific interactions (Chapter 9). Receptors for ligands may be localized in tissues or cells by modification of the ligand with the appropriate fluorophore. Targeted molecules may be... 

In order to facilitate analysis of FeBABE produced fragments, the prey protein or biomolecule is labeled at one end with a tag that can be detected after electrophoresis, usually in a transfer blot. The tag can be a fusion tag, such as 6X His, or any other group that can be targeted with an antibody and detected. Alternatively, radiolabels and fluorescent labels have been used with prey molecules, including the use of end-labeled DNA to study where DNA binding proteins dock onto the oligonucleotide sequence. 

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