Oligodeoxyribonucleotides purification

As mentioned in last year s Report, aromatic phosphoramidates have been used to protect 5 -phosphoryl groups in the stepwise synthesis of oligodeoxyribonucleotides. The appropriate monomer units are coupled with DCC and the phosphoramidate protecting group is removed when required with isoamyl nitrite. A rapid and general preparative method for oligonucleotides has been developed based on phosphoramidates of the highly lipophilic 4-aminophenyltriphenylmethane (25). Purification of... 

Puma, P., Duffey, D., and Dawidczyk, P., U.S. Patent appl. 94,944, Purification of oligodeoxyribonucleotide phosphorothioates using DEAE-5PW anion ion-exchange chromatography and hydrophobic interaction chromatography, 1994. 

Sawai, H., Analysis and purification of synthetic large oligodeoxyribonucleotides by HPLC on RPC-5 like resin, Nucleic Acids Res., 17, 113, 1986. 

Zon, G. (1990). Purification of synthetic oligodeoxyribonucleotides. In High-performance Liquid Chromatography in Biotechnology (W. Hancock, ed.), pp. 301-397. Wiley, New York. 

Zon, G., and Thompson, J. A. (1986). A review of high-performance liquid chromatography in nucleic acids research. II. Isolation, purification and analysis of oligodeoxyribonucleotides. Bio Chromatography 1, 22-32. 

Green, A. P., Burzynski, J., Helveston, N. M., Prior, G. M., Wunner, W. H., and Thompson, J. A. (1995). HPLC purification of synthetic oligodeoxyribonucleotides containing base- and backbone-modified sequences. BioTechniques 19, 836-841. 

Reverse-phase h.p.l.c. has been demonstrated to be effective for the purification of very long (88-143 bases) 5 -0-dlmethoxytrityl-derlvatised oligodeoxyribonucleotides. The isolated yields were superior to those obtained using electrophoresis followed by electroelution. A fast procedure has been reported for the purification of oligodeoxyribonucleotides of varying... 

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