Ochrosia elliptica Ellipticine

Ellipticine (1) and 9-methoxyellipticine (2) have been isolated from Ochrosia acuminata stems (22) and from in vitro callus cultures derived from the stems of Ochrosia elliptica (23,24). It is found that the in vitro production of these alkaloids can be increased by cloning small cell aggregates. 

A fluorodensitometric assay was developed by Montague and co-workers 140) to analyze cultures from Ochrosia elliptica for ellipticine (1), 9-methoxy-ellipticine (2), and 9-hydroxyellipticine (3) by thin-layer chromatography (TLC) without the need for prior purification. Using silica gel impregnated with dimethyl sulfoxide and a mobile phase of EtOAc-water-l-octanol (17 2 2), these workers were able to achieve good separation of these alkaloids and to assay the resulting chromatograms by fluorodensitometry (40-300 fmol detection limits of alkaloid). 

Ellipticine 9-Hydroxyellipticine Ochrosia elliptica Ochrosia elliptica 3940.00 4680.00 O. elliptica SC 0.005% DW... 

Ellipticine (41) and its 9-methoxy derivative (42) have great interest as antitumor dmgs. These alkaloids are isolated from the leaves of Ochrosia elliptica, a shmb found on scattered islands in the Indian and Pacific Oceans. Plant cell cultures would thus be of interest as an alternative means of production of the antitumor alkaloids. For a review on the genus, its alkaloids, and cell culture, the reader is also refered to Chenieux et al. (716). 

Ochrosia elliptica Catharanthine Vindoline Vinblastine Vincristine Vindesine Alioline Ellipticine... 

Olivacine (52) occurs in the bark of several Brazilian trees of the genera Aspidosperma and Tabernaemontana (Apocynaceae) ellipticine (53) and 9-methoxyellipticine (54) have been isolated from species of Ochrosia (Apocynaceae) (Neuss, 1980). Similar alkaloids have been isolated from Strychnos dinklagei (Gribble, 1990). Both ellipticine and 9-melhoxyellipticine (54) have been isolated at low levels from callus cultures of Ochrosia elliptica (Gribble, 1990). 

Ochrosia elliptica (Apocynaceae) is a small tropical evergreen tree, which contains ellipticine in its leaves and stems. Ellipticine has also been isolated from Ochrosia sandwicensis, O, viellardii, O. silvatica (28, 53, 85), and Aspidosperma subin-canum (50). 

A number of species of the genus Ochrosia have been investigated, and among the alkaloids already discussed are ellipticine and methoxy-ellipticine from 0. elliptica (Section VII, D). Other alkaloids from this species include elliptinine for which structure CCCLVIII has been... 

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