Ochrobactrum anthropi NCIMB

Sonke T, S Ernste, RF Tandler, B Kaptein, WPH Peeters, FBI van Assema, MG Wubbolts, HE Schoemaker (2005) L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321. Appl Environ Microbiol 71 7971-7963. 

A. L. L. Duchateau, and J. Kamphuis, Ochrobactrum anthropi NCIMB 40321 a new biocatalyst with broad-spectrum L-specific amidase activity, Appl. Microbiol. Biotechnol. 1993, 39, 296-300. 

An amidase from Ochrobactrum anthropi strain NCIMB 40321 has a wide snbstrate versatility for L-amides, primarily those with an a-amino gronp (Sonke et al. 2005), while the condensation prodnct of nrea and formaldehyde H2N-[CONH-CH2NH] -CONH2 is hydrolyzed by another strain of 0. anthropi (Jahns et al. 1997). 

By this procedure three microorganisms could be identified that hydrolyzed DL-mandelic acid amide completely L-enantiospecific. The most interesting one of these three strains, based on its high cellular activity, could be identified as an Ochrobactrum anthropi strain, and this strain has been deposited at the NCIMB culture collection as NCIMB 40321. 

---