O-serotyping schemes

The development of an O serotyping scheme is complex. Heated O antigen preparations of unknown cultures are tested first for agglutination on slides with droplets of pooled, polyvalent O antisera. If agglutination occurs with one of the pooled antisera, the heated suspensions are then tested for agglutination using the individual O antisera contained in that pool. Next, for positive reactions, the heated... 

Pedersen, K., Grisez, L., R. van Houdt, Tiainen, T., Ollevier, F., Larsen, J.L. Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O-serogroups. Curr Microbiol 38 (1999) 183-189. 

Penner, J.L., Hennessy, J.N. Separate O-grouping schemes for serotyping clinical isolates of Proteus vulgaris and Proteus mirabilis. J Clin Microbiol 12 (1980) 304-309. 

The disaccharide epitope a-Tyv-(l —>3)-a-o- Man present in the D1 Salmonella serotype was synthesized as the pentenyl glycoside. The tyvelose donor 138 was prepared from ethyl l-thio-p-o-mannopyranoside 137 employing a single dideoxygenation step (Scheme 40). Glycosylation of 138 with the mannopyranosyl... 

Defragmentation and activation could be performed simultaneously. For example, partial de-iV-acetylation and nitrous acid deamination of the Sp serotype 14 CPS [98] proceeds with a rearrangement leading to a terminally activated oligo or polysaccharide according to the O Scheme 1. 

The infections caused by Plesimonas shigelloides, a gram-negative bacterium, strongly correlate with the surface water contamination and are particularly common in tropical and subtropical habitats. The structure of the O-specific side chain of P. shigelloides LPS (strain CNCTC 113/92, serotype 54) has been recently elucidated and revealed a novel hexasaccharide repeating unit (Scheme 2.28) containing two unusual 3-linked heptose units, one of which is 6-deoxy [58]. In... 

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