Nutrient medium Streptomycin

AR42J and HT-29 cells were cultured in Ham s F12K nutrient medium supplemented with 5% foetal bovine serum, 100 lU/mL penicillin and 50 pg/ mL streptomycin. MCF-7 cells were cultured in Dulbecco s modified Eagle s medium (DMEM) containing 10% foetal bovine serum, 100 lU/mL penicillin, 50 pg/mL streptomycin and lOmM glutamine. All cell cultures were grown to confluence in a humidified atmosphere with 5% CO, at 37°C. Trypsin-EDTA solution was used for subculturing and cell isolation. 

Linoleic acid and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma Chemical Co. (St. Louis, MO). Dulbeccos Modified Eagle s Medium/Hams F-12 nutrient mixture (DMEM/F12) containing 2 pM linoleic acid, fetal bovine serum (FBS), trypsin-EDTA, penicillin-streptomycin, selenium and transferrin were purchased from Gibco BRL (Rockville, MD). Other chemicals used were of reagent grade. 

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