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Nutmeg, lipid extract

Lipids, relatively nonpolar chemical substances found in plant, bacterial, and animal cells, are among the most ubiquitous of biomolecules. In this experiment, a lipid extract of ground nutmeg will be purified by chromatography on a silica gel column. Analysis of the lipid extract by thin-layer chromatography will provide the classification of the components in the extract. The unknown lipids will be further characterized by saponification and analysis of the fatty acid content by gas chromatography. For an abbreviated experiment, students may be provided samples of natural oils and fats that can be analyzed by saponification and gas chromatography. [Pg.303]

Calculate the approximate percentage lipid composition of the nutmeg (assume the lipid recovery is quantitative). Prepare a table listing the R values obtained for each standard lipid and for the crude and purified unknown. By comparing R values, identify the general class of lipid to which your purified extract belongs. How effective was your purification step If... [Pg.317]

B 8. What is the identity of the lipid that was extracted from nutmeg seed B 9. What is the function of BF3 in the formation of FAMEs ... [Pg.318]

Dl.l Extraction and Measurement of Total Lipids Basic Protocol 1 Solvent Extraction of Oilseeds, Nutmeg, and Other Foods Dl.1.1... [Pg.423]

The ethanolic extract of nutmeg kernels showed hypolipidaemic effect in albino rabbits. Administration of 500mg/kg of the extract daily for a period of 60 days in the hyperlipidaemic rabbits resulted in significantly lower levels of lipoprotein lipids (total cholesterol 574 61 versus 210 27mg/dl low-density lipoprotein (LDL) cholesterol 493 57 versus 131 25mg/dl and triglycerides 108 14 versus 67 in control versus experimental) (Ram et al., 1996). [Pg.181]

Spricigo, C.B., Pinto, L.T., Bolzan, A. and Novais, A.F. (1 999) Extraction of essential oil and lipids from nutmeg by liquid carbon dioxide. Journal of Supercritical Fluids 15, 253-259. [Pg.188]

What IS the identity of the lipid that was extracted from nutmeg seed ... [Pg.324]

A yield of about 16% is obtained from nutmeg kernals by L.CO2, the extract obtained containing 40% by weight of low molecular weight saturated and stable lipids [22, 161]. [Pg.172]

See other pages where Nutmeg, lipid extract is mentioned: [Pg.308]    [Pg.308]    [Pg.168]    [Pg.314]    [Pg.96]    [Pg.306]    [Pg.306]    [Pg.181]    [Pg.181]    [Pg.243]    [Pg.191]    [Pg.312]   
See also in sourсe #XX -- [ Pg.303 , Pg.304 , Pg.305 , Pg.306 , Pg.307 , Pg.308 , Pg.309 , Pg.310 , Pg.311 , Pg.312 , Pg.313 , Pg.314 , Pg.315 , Pg.316 , Pg.317 , Pg.318 ]

See also in sourсe #XX -- [ Pg.303 , Pg.304 , Pg.305 , Pg.306 , Pg.307 , Pg.308 , Pg.309 , Pg.310 , Pg.311 , Pg.312 , Pg.313 , Pg.314 , Pg.315 , Pg.316 , Pg.317 , Pg.318 ]

See also in sourсe #XX -- [ Pg.303 , Pg.304 , Pg.305 , Pg.306 , Pg.307 , Pg.308 , Pg.309 , Pg.310 , Pg.311 , Pg.312 , Pg.313 , Pg.314 , Pg.315 , Pg.316 , Pg.317 , Pg.318 ]



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