Nucleic acid hybridization interaction

The difficulty with protein arrays is that proteins do not behave as uniformly as nucleic acid. Protein function is dependent on a precise, and fragile, three-dimensional structure that may be difficult to maintain in an array format. In addition, the strength and stability of interactions between proteins are not nearly as standardized as nucleic acid hybridization. Each protein-protein interaction is unique and could assume a wide range of affinities. Currently, protein expression mapping is performed almost exclusively by two-dimensional electrophoresis and mass spectrometry. The development of protein arrays, however, could provide another powerful... 

Nucleic acid structures and sequences primary and secondary structure of DNA fragments, translocation of genes between two chromosomes, detection of nucleic acid hybridization, formation of hairpin structures (see Box 9.4), interaction with drugs, DNA triple helix, DNA-protein interaction, automated DNA sequencing, etc. 

This chapter presented the results of research aimed at the combined use of transition metal coordination and nucleic acid hybridization to construct hybrid inorganic-nucleic acid supramolecular structures. The interaction of alkali metal ions with DNA and RNA is nonspecific, and has been long... 

The interactions of nucleic acid materials with Ln have been largely studied through their luminescent properties. The known affinity of Ln " cations for the phosphate moiety has led to many investigations of the Ln interactions with nucleotides, covered in some detail by Evans [1]. The interaction of Ln with nucleic acids has been used to probe perturbations in the integrity of nucleic acid strands and as an assay of nucleic acid hybridization. Balcarova and Brabec [45] showed that the interaction of Tb with double-stranded DNA can be used to monitor guanine bases present in distorted double-stranded regions of DNA. The enhancement of distortions in double-stranded DNA appears to be restricted to certain modifiers of nucleic acid as there is no... 

Along with its robust rod-shaped dimensions, TMV s simple structure and disassembly mechanism have presented a unique opportunity to create viral arrays via nucleic acid hybridization. Specifically, the binding force between the genomic RNA and coat proteins is weakest at TMV s 5 -end where a change in pH and Ca + concentration in the intracellular environment of host cells results in a destabilization of the interaction between the 5 -end of the RNA and coat proteins. This induces partial disassembly of the coat proteins so that the 5 -end RNA sequence is presented to the host s ribosome, which initiates translation of the viral protein replicase. By mimicking this condition via ultracentrifugation at high pH, the... 

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