Nuclear magnetic resonance structure verification

Chapter 10, on nuclear magnetic resonance spectroscopy was included because of the unique role that nuclear magnetic resonance spectroscopy has in the study of polymorphisms as well as in verification of compound identity and the structural confirmation of metabolic or decomposition products of drugs. Chapter 11, Vibrational Spectroscopy, reflects the invaluable contributions that those techniques such as infrared spectroscopy have made to the analysis of formulations, among other studies. 

The fidelity of the protein-based polymer expression from a prepared gene is extraordinary when compared to the chemically prepared product. Even so, it is important to use purification procedures that limit alteration of the structure and to establish that proteolytic or other enzymatic activity is sufficiently limited that the desired properties of the expressed protein-based polymer are retained. An important method with which to achieve adequate verification of the gene product again utilizes multi-dimensional nuclear magnetic resonance. 

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