Two-dimensional nuclear magnetic

Boelens, R., et al. Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance. /. Mol. Biol. 193 213-216, 1987. 

A. Bax, Two-Dimensional Nuclear Magnetic Resonance in Liquids, Reidel, Dordreeht, 1984. 

Giusti, M.M., Ghanadan, H., and Wrolstad, R.E., Elucidation of the structure and conformation of red radish Raphanus sativus) anthocyanins using one- and two-dimensional nuclear magnetic resonance techniques, J. Agric. Food Chem., 46, 4858, 1998. 

Servili M, Baldioli M, Selvaggini R, Macchioni A and Montedoro GF. 1999. Phenolic compounds of olive fruit one and two-dimensional nuclear magnetic resonance characterization of niizhenide and its distribution in the constitutive parts of fruit. J Agric Food Chem 47(1) 12—18. 

The structures of vanicosides A (1) and B (2) and hydropiperoside (3) were established primarily by one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy techniques and fast atom bombardment (FAB) mass spectrometry (MS).22 The presence of two different types of phenylpropanoid esters in 1 and 2 was established first through the proton (4H) NMR spectra which showed resonances for two different aromatic substitution patterns in the spectrum of each compound. Integration of the aromatic region defined these as three symmetrically substituted phenyl rings, due to three p-coumaryl moieties, and one 1,3,4-trisubstituted phenyl ring, due to a feruloyl ester. The presence of a sucrose backbone was established by two series of coupled protons between 3.2 and 5.7 ppm in the HNMR spectra, particularly the characteristic C-l (anomeric) and C-3 proton doublets... 

Fig. 3. (Top) A two-dimensional nuclear magnetic resonance spectrum of a sugar (Bottom) conventional NMR spectrum of same sugar. Tile two-dimensional spectrum also can be plotted as a contour map with intensities denoted by color. (JEOL Inc)...

Bain, L. MRI—Safety Issues Stimulate Concern," Science. 1245 (May 31, 1991). Bax, A. and L. Lerner Two-Dimensional Nuclear Magnetic Resonance Spectroscopy, Science, 232, 960-967 (1986). 

Andersen, 0.M., Opheim, S., Aksnes, D.W., and Frpystein, N.A. 1991b. Structure of petanin, an acylated anthocyanin isolated from Solanum tuberosum, using homo- and hetero-nuclear two-dimensional nuclear magnetic resonance techniques. Phytochem. Anal. 2 230-236. 

Kosir, I. and Kidric, J. (2001). Identification of amino acids in wines by one- and two-dimensional nuclear magnetic resonance spectroscopy. ]. Agric. Food Chem. 49, 50-56. 

Haiber, S., Burba, R, Herzog, H., and Fambert, J. (1999). Elucidation of aquatic humic partial structures by multistage ultrafiltration and two-dimensional nuclear magnetic resonance spectrometry. Fresenius J. Anal Chem. 364, 215-218. 

Simpson, A. J., Salloum, M. J., Kingery W. L., and Hatcher, P. G. (2002b). Improvements in the two-dimensional nuclear magnetic resonance spectroscopy of humic substances. J. Environ. Qual. 31, 388-392. 

Eriksson, M., Leijon, M., Eiiort, C., Norden, B. and Graslund, A. (1992) Minor groove binding of [Ru(phen)3]2+ to [d(CGCGATCGCG)]2 evidenced by two-dimensional nuclear magnetic resonance spectroscopy. J. Am. Chem. Soc., 114, 4933-4934. 

By observing sequential neutral losses, further information is obtained to determine the sequence of substructures or molecular connectivity within the analyte (Lee et al., 1996). This procedure is analogous to two-dimensional nuclear magnetic resonanse (NMR) techniques used to sequentially connect substructures. A familiar example of molecular connectivity is the determination of the amino acid sequence of a peptide. Specific neutral losses are diagnostic of specific amino acids, and the sequence of these losses identifies the peptide (Roepstorff and Fohlman, 1984). 

J. L. Markley, Methods Enzymol., 176, 12 (1989). Two-Dimensional Nuclear Magnetic... 

Bax A. Two-Dimensional Nuclear Magnetic Resonance in Liquids. Delft University Press, D. Reidel Publishing Co 1982 (especially pp. 12-23, introduction, pp. 129-153, multiple quantum coherence, and pp. 188-200, density matrix). 

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