Nuclear magnetic resonance high-throughput

Hajduk PJ, Gerfm T, Boehlen JM et al (1999) High-throughput nuclear magnetic resonance-based screening. J Med Chem 42 2315-2317... 

Vinarov, D.A. and Markley, J.L. (2005) High-throughput automated platform for nuclear magnetic resonance-based structural proteomics. Expert Review of Proteomics, 2 (1), 49-55. 

Nuclear magnetic resonance (NMR) measurements are usually considered to be slow processes, but recent advances in the design of flow-through NMR cells have allowed the method to be applied in combinatorial chemistry 97). These technological improvements were applied to the development of two NMR-based high-throughput ee assays for evaluating the products of enzyme- or transition metal-catalyzed reactions 98). 

High-resolution nuclear magnetic resonance spectroscopy also has the potential for rapid sample throughput and could expand the range of metabolites readily detectable. The instrumentation is expensive and interpretation requires considerable... 

In general, however, detailed protocols for the direct analysis of stereochemical features by mass spectrometric means have not been achieved so far. Instead, other analytical methods, such as X-ray analysis or nuclear magnetic resonance (NMR), elegantly address these issues [3]. However, with respect to the analysis of trace compounds and in the context of high-throughput analytical methods, it would be beneficial to develop tools for rapid stereochemical assays by mass spectrometric means or hyphenated techniques [4,5]. 

The demand for enzyme assays that not only monitor overall activity but also en-antioselectivity stimulated the development of further assay systems that are still, however, in a rather experimental state with respect to high-throughput enzyme screening applications. These methods include assays based on electron spin resonance spectroscopy (ESR) [91], nuclear magnetic resonance (NMR) [92,93], IR-thermography [94] or electrospray ionization spectrometry (ESI-MS) [95]. 

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