NS0 cells

Although numerous cell lines have been screened for their efficiency as a host system for recombinant protein production, only a few have shown favorable properties for the expression of biopharmaceuticals (Hauser, 1997). Regulatory and economic issues for large-scale production and the intended application of the recombinant protein (diagnosis, therapy, etc.) have to be carefully considered (Makrides and Prentice, 2003). Three mammalian cell lines are now commonly used by the pharmaceutical industry Chinese hamster ovary (CHO) cells, the murine myeloma SP2/0 and the NS0 cell line (see Table 3.1). These cell lines have been used to produce 11 of 21 therapeutic products approved from 1996 to 2000 (Chu and Robinson, 2001). 

Tey BT, Al-Rubeai M (2004), Suppression of apoptosis in perfusion culture of myeloma NS0 cells enhances cell growth but reduces antibody productivity, Apoptosis 9 843-852. 

Dempsey, J., S. Ruddock, M. Osborne, A. Ridley, S. Sturt, and R. Field. 2003. Improved fermentation processes for NS0 cell lines expressing human antibodies and glutamine synthetase. Biotechnol Prog 19 175-178. 

Several different cell lines have already been investigated in perfusion cultures using the acoustic cell filter. For instance, Shirgaonkar et al. (2004) used NS0, HEK-293, SP2-derived hybridoma, and insect cells in different serum-supplemented and serum-free media at different perfusion rates and acoustic chamber volumes. According to these authors, an adequate operation of the filter depends on optimization of operational... 

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