Non-Leloir pathways

Both transferases from the Leloir and non-Leloir pathways have been employed for the in vitro synthesis of oligosaccharides and glycoconjugates. Glycosidases have also been exploited for synthesis. The function of glycosidases in vivo is to cleave glycosidic bonds,... 

Glycosyl transferases are essential enzymes for oligosaccharide biosynthesis. These enzymes can be classified as enzymes of the Leloir pathway and those of the non-Leloir pathway. The glycosyltransferases of the Leloir pathway are involved in the biosynthesis of most iV-linked... 

A variety of linear oligo- and polysaccharides can be also prepared using glycosyltransferases of the non-Leloir pathway, which utilize sugar z-phosphates as... 

The use of the non-Leloir pathway enzymes, which require sugar-l-phosphates as donors. 

It is clear, then, that perhaps no other group of compounds is as well suited to enzyme-based synthesis as is the carbohydrates. During the past several years, we have developed a range of enzyme-based syntheses for mono-, oligo-, and polysaccharides. This paper reviews some of our efforts on the use of aldolases and transketolase for monosaccharide synthesis, as well as the use of Leloir and non-Leloir pathway enzymes for glycosidic bond formation. 

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