Nitroxide compounds model

Attachment of B ansformation Products of Stabilizers. Up-to-date knowledge dealing with the chemistry of transformation products of phenolic [6, 15, 17, 20] and aromatic aminic [16, 43, 230] antioxidants and photoantioxidants based on hindered piperidines [10] indicates the possibility of attaching compounds having structures of quinone imine or quinone methide, or of radical species like cyclohexadienonyl, phenoxyl, aminyl or nitroxide to polymeric backbones. These reactions proceed mostly via reactivity of macroalkyl radicals derived fi-om stabilized polymers. Various compounds modelling this reactivity have been isolated [19, 230]. These results are of importance mainly for the explanation of mechanisms of antioxidant activity [6, 22, 24]. 

Steenken S, Jagannadham V (1985) Reaction of 6-yl radicals of uracil, thymine, and cytosine and their nucleosides with nitrobenzenes via addition to give nitroxide radicals. OH catalyzed ni-troxide heterolysis. J Am Chem Soc 107 6818-6826 Steenken S, Jovanovic SV (1997) How easily oxidizableis DNA One-electron reduction potentials of adenosine and guanosine radicals in aqueous solution. J Am Chem Soc 119 617-618 Steenken S, Behrens G, Schulte-Frohlinde D (1974) Radiation chemistry of DNA model compounds, part IV. Phosphate ester cleavage in radicals derived from glycerol phosphates. Int J Radiat Biol 25 205-210... 

To realize the above-mentioned systems, we carefully chose suitable switching units and radical moieties. As an initial attempt, we employed l,2-bis(2-methyl-l-benzothiophen-3-yl)perfluorocyclopentene (9a) as a photochromic spin coupler (Scheme 9.2). Compound 9a is one of the most fatigue-resistant diarylethenes [21]. Nitronyl nitroxide was chosen for the spin source, because this radical is jr-conjugative. Thus, we designed molecule 10a, which is an embodiment of the simplified model 8a [37, 62]. 

Table 1 displays rate data for alkoxyamine-termi-nated polymers and low molecular model compounds and shows some important trends. At about the same temperature, the dissociation rate constants Ad of alkoxyamines (Schemes 12 and 30) with the same leaving radical (polystyryl, 1-phenylethyl) increase in the order 3 (TEMPO) < 6 < 8 (DEPN) < 1 (DBNO) by a factor of about 30. Acrylate radicals dissociate markedly slower than styryl radicals from 1 (DBNO), but there is no appreciable difference for 8 (DEPN). The dependence of Ad on the nitroxide structure has been addressed by Moad et al.104 They found the order five membered ring < six membered ring < open chain nitroxides and pointed out additional steric (compare 3 and 6) and polar effects. 

The kinetic parameter for the radical dissociation of a carbon—halogen terminal was obtained with the use of an isolated polystyrene with a terminal C—Br bond in the presence of a copper catalyst and a conventional radical initiator with a long half-life.282-283 The result was compared with that of low molecular weight compounds of similar carbon-halogen bonds.163 The second-order rate constant of the model compound 1-13 (X = Br), an effective initiator for styrene, is comparable to that of the polymer terminal. Alternatively, rate constants can be obtained by using a combination of nitroxide-exchange reactions and HPLC analysis.242... 

The presence of multiple spins in the same protein or protein complex creates interpretative problems in DEER, due to the appearance of artifact peaks in the distance distribution. Methods to identify artifact distances in multispin systems are under development [72]. Strategies to overcome problems due to the superposition of distances in the same polypeptide can make use of orthogonal labeling (e.g., using different spin labels binding to cys and unnatural amino acids [73]). In the easier case of protein complexes with spin labels in different subunits, one can use different nitroxide isotopes, as demonstrated on model compounds [74]. 

One-bond carbon-nitrogen couplings, Dcn of ca. 3 Hz have been measured by Kelemen et aU for a series of labelled alkoxyamines, the model compounds for nitroxide-trapping studies in free radical copolymerization. Absolute Dcn values have been obtained for some mesoionic compounds and related structures by Jazwinski and Staszewska-Krajewska. " ... 

The [(acac)2Cu-ONH2] model complex, shown in Figure 7, mimics a classical series of compounds containing a transition metal ion (i.e. Cu(II)) bound to a nitroxide radical. When the nitroxide radical is bound to the metal in axial position a triplet is found to be the ground state independently on the value of the migles 0 and /. The actual value of J is related only to the nature of the nitroxide. [50]. 

The stereospecificity of the interactions of several spin-labelled substrates with cyclohexa- and cyclohepta-amyloses, as models for chymotrypsin, has been studied. Complexes of the cycloamyloses with 2,2,6,6-tetramethyl-4-oxy-pyridyl-1-oxide in aqueous solution were examined by e.s.r. spectroscopy the nitroxide function moved to a relatively hydrophobic environment on binding to cyclohepta-amylose, and lost some freedom of rotation on binding to both cycloamyloses. The dissociation constant for the cyclohexa-amylose complex of the nitroxide is greater than that for the cyclohepta-amylose complex, consistent with measurements made on molecular models. In the hydrolysis of the asymmetric compound 3-carboxy-2,2,5,5-tetramethylpyrrolidyl-l-oxide 3-nitro-phenyl ester, catalysed by cyclohexa-amylose, enantiomeric specificity was observed in the acylation step but not in formation of the Michaelis complex , or on hydrolysis of the acylated cycloamylose intermediate. No differences were found in the e.s.r. spectra of solutions of the trapped acylcyclohexa-amylose intermediates derived from ( + )- and ( )-forms of the asymmetric nitroxide. The nitroxide function is less free to rotate in the acylcycloamylose intermediate than in the Michaelis complex and is not included in the cycloamylose cavity. 

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