Nitroso compounds, detection identification

The first observations of the additions of transient radicals to nitroso compounds (equation 60) and nitrones (equation 61) to form stable nitroxyl radicals, which could be conveniently detected by ESR for the identification of the transient radicals, were reported in the mid-1960s. This technique has been extensively applied in chemical "" and biological systems. " ... 

Spin traps come in basically two types nitroso compounds and nitrone compounds. Reactive free radicals react with the carbon of the nitrone functional group to form a radical adduct that always has a nitroxide group, which is an unusually stable type of free radical. Nitrones are the most useful spin traps for the in vivo detection of free radical metabolites because of the stability of the resulting radical adduct. However, identification of the parent radicals can be difficult because adducts derived from different radicals often have very similar EPR spectra. A comprehensive review of this area through 1992 has recently been published [48]. 

Trapping free radicals by the spin trapping technique. This technique is used for the detection and identification of short-lived free radicals by detecting the ESR spectra of nitroxyl addition products (spin adducts) of free radicals (R ) to nitroso (e.g. 2-nitroso-2-methyl propane, t-butyl nitroxide 10.19), nitrobenzene (10.20), pentamethylnitrobenzene (10.21)) and nitrone (phenyl-N-tert-butyl nitrone) (10.22) compounds called spin traps ... 

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