Nitroblue tetrazolium chloride

Alkaline phosphatase 5-Bromo-4-chloro-3 -indolyl-phosphate with nitroblue tetrazolium chloride (BCIP/NBT) Black/purple... 

Glucose oxidase Phenazine methosulfate with nitroblue tetrazolium chloride Black/purple... 

Figure 6-17. Reactions involved in coupling oxidation of lactate with the reduction of p-nitroblue tetrazolium chloride.

Fig. 2. Typical densitometnc scan. Calf thymus DNA, methylated in vitro with 5 mM of methylnitrosourea, was denatured in 50 mM sodium hydroxide and blotted onto nitrocellulose. After incubation with a rabbit antiserum specific for O -methyldeoiQrguanosine (NPZ-193-1,1 8,000 see ref. 4) and alkaline phosphatase conjugated goat-antirabbit IgG, the blot was developed with 5-bromo-4-chloro-3-indolylphosphate toluidine salt and nitroblue tetrazolium chloride in diethanolamine buffer, as described in the Methods section, and scanned by reflectance densitometry at 530 nm. Slots contain 283, 94.7, 31.3, 10.4, 3.4, 1.1, and 0 fmol of 0 -methyl-deoxyguanosine, corresponding to 181-0.7 pmol/mol of deoxyguanosine.

Substrate solution (16) Dissolve nitroblue tetrazolium chloride at a Hnal concentration of 0.3 mg/mL in diethanolamine buffer. Immediately before use, add 3.4 lL/mL of the BCIP soludon and mix well. Keep away from direct light. 

Nitroblue tetrazolium chloride T ris(hydroxymethyl)aminomethane T-suppressor cells International units (in enzymology) Ultraviolet... 

The nitroblue tetrazolium assay (111) is another indirect method that is used especially for detecting SOD activity on gel electrophoresis. Superoxide radicals are generated by xanthine/xanthine oxidase or by the photoreduction of flavins (typically riboflavin), which oxidize H2O to O2. The gel on which SOD samples have been loaded is then stained with nitroblue tetrazolium chloride. This reagent is reduced by superoxide to the blue-colored formazan. SOD competes with nitroblue tetrazolium and produces colorless zones on the blue gels. This method, which is highly speciflc toward superoxide dismutase, is limited by its low reliability with respect to quantitative determinations. 

Nitroblue tetrazolium chloride (NBT) Dissolve 35 mg NBT in 277 pL 70% dimethylformamide (DMF). Prepare freshly before use. 

EINECS 206-0674 NBT NBT (dye) Nitro Blue tetrazolium salt Nitro Blue tetrazolium chloride Nitro BT Nitro tetrazolium BT Nitroblue tetrazolium Nitroblue tetrazolium salt (4-Nitrophenyl)-5-phenyl-,dichloride Nitrotetrazolium Blue Nitrotetrazolium Chloride Blue NSC 27622 p-Nitro Blue tetrazolium p-Nitro Blue tetrazolium chloride p-Nitrotetrazolium Blue 2H-Tetrazolium, 3,3 -(3,3 -dimethoxy(1,1 -biphenyl)4,4 -diyl)bis(24,4 -ylene)ditetrazolium dichloride Tetraz-olium nitro BT Tetrazolium Nitro Blue. Activity slain for electrophoresis and histochemistry. Substrate for dehydrogenase. Crystals mp = 200". Monomer-Polymer Dajac. 

The undamaged myocardium contains dehydrogenase and substrate which are capable of reducing the faintly yellowish tetrazolium salt nitroblue tet-razolium (formula [18]) to dark blue formazan. Sandritter and Jestadt (1957) and Jestadt and Sandritter (1959) used 2,3,5-triphenyltetrazolium chloride (formula [17]) in human material and rabbits after ligation of the Ramus descendens of the left coronary artery for microscopic identification of fi esh myocardial infarction, while Nachlas and Shnitka (1963), Andersen and Hansen (1973) and Nitz etal. (1982) utiUsed nitroblue tetrazolium. 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetra-zoUum bromide also gives a blue formazan (Jestadt and Sandritter 1959). 

Other Names 2//-Tetrazolium, 2,2 -(3,3 -dimethoxy [l,l -biphenyl]-4,4 -diyl)bis[3,5-bis(4-nitrophenyl)-, dichloride 2//-Tetrazolium, 3,3 -(3,3 -dimethoxy-4,4 -bi-phenylylene)bis[2,5-bis(p-nitrophenyl)-, dichloride [3,3 -(3,3 -Dimethoxy-4,4 -biphenylylene)bis[2,5-bis(p-nitrophenyl)-2//-tetrazolium chloride]] 2,2, 5,5 -Tetra-p-nitrophenyl-3,3 -(3,3 -dimethoxy-4,4 -biphenylene)dite-trazolium chloride 2,2, 5,5 -Tetrakis(p-nitrophenyl)-3,3 -(3,3 -dimethoxy-4,4 -diphenylene)ditetrazolium chloride 2,2, 5,5 -Tetrakis-p-(nitrophenyl)-3,3 -bis(3,3 -di-methoxy-4,4 -diphenylene)ditetrazolium chloride Blue p-nitrotetrazolium chloride NSC 121208 Nitroblue monotetrazolium chloride TNBT Tetranitro Blue Tetrazolium Tetranitro-BT Tetranitroblue tetrazolium chloride Tetranitrotetrazolium blue Merck Index Number Not listed... 

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