Nitric oxide measurement

T. Malinski, F. Bailey, Z.G. Zhang, and M. Chopp, Nitric-oxide measured by a porphyrinic microsensor in rat-brain after transient middle cerebral-artery occlusion. J. Cereb. Blood Flow Metab. 13, 355-358 (1993). 

K. Ichimori, H. Ishida, M. Fukahori, H. Nakazawa, and E. Murakami, Practical nitric-oxide measurement employing a nitric oxide-selective electrode. Rev. Sci. Instrum. 65, 2714-2718 (1994). 

Y. Kitamura, T. Uzawa, K. Oka, Y. Komai., H. Ogawa, N. Takizawa, H. Kobayashi, and K. Tanishita, Microcoaxial electrode for in vivo nitric oxide measurement. Anal. Chem. 72, 2957-2962 (2000). 

Rate of biological inactivation of nitric oxide. The apparent first-order rate constants were calculated as the natural logarithm of two divided by the half-life of nitric oxide measured in perfusion cascades. The half-lives were 30 sec with room air and 6-8 sec with 95% oxygen (Furchgott and Vanhoutte, 1989). Under anaerobic conditions, nitric oxide is indefinitely stable. The slope of the line give a second-order rate constant for the inactivation of nitric oxide by oxygen as approximately 100 M" sec". ... 

Destruction of nitric oxide by superoxide in the buffers is more likely to account for the short half-life of nitric oxide in vitro. Superoxide dismutase (15-100 U/ml) substantially increased the apparent half-life of EDRF, strongly suggesting that superoxide contributes to the short biological half-life of nitric oxide. In the perfusion cascade bioassay system, the buffers are bubbled with 95% oxygen, contain 11 mM glucose as well as trace iron plus copper contamination and are incubated under the weak ultraviolet (UV) radiation of fluorescent lights. These are prime conditions for the autoxidation of glucose to form small amounts of superoxide in sufficient amounts to account for the short half-life of nitric oxide in nanomolar concentrations. The rate of reaction between superoxide and nitric oxide is 6.7 X 10 M sec L The shortest half-life of nitric oxide measured is approximately 6 sec. To achieve a half-life of 6 sec, the steady state concentration of superoxide would only need to be 17 pM, calculated as ln(2)/ (6 sec X 6.7 X 10 M" sec )-... 

McFarland M., Kley D., Drummond J.W., Schmeltekopf A.L. and Winkler R.H., Nitric oxide measurements in the equatorial Pacific region. Geophys. Res. Lett., 6, 605-608 (1979). 

Persson, M.G., Zetterstrom, O., Agrenius, V. et al. (1994). Single-breath nitric oxide measurements in asthmatic patients and smokers. Lancet 343, 146-147. 

Solomon, S.C., C.A. Barth, and S.M. Bailey, Auroral production of nitric oxide measured by the SNOE satellite. Geophys Res Lett 26, 1259, 1999. 

Fahey, D.W., S.R. Kawa, and K.R. Chan, Nitric oxide measurements in the arctic winter stratosphere. Geophys Res Lett 17, 489, 1990a. 

Baukal, C. E., and Dalton, A. I. "Nitric Oxide Measurements in Oxygen Enriched Air-Natural Gas Gombustion Systems." Proceedings of the Fossil Fuel Combustion Symposium, edited by S. N. Singh, New York ASME, 1990. 

Bellamy TC, Griffiths C, Garthwaite J (2002) Differential sensitivity of guanylyl cyclase and mitochondrial respiration to nitric oxide measured using clamped concentrations. J Biol Chem 277 31801-31807... 

Figure 1. Nitric oxide measurement ( jmoi/mi) in cuiture medium in static versus dynamic conditions, 24 and 48 hr after seeding MC3T3 on the devices, and immediately after stimulation at 1 and 3 Hz. NO values are significantly higher in the dynamic group.

Malinski T, Mesaros S, Tomboulian P (1996) Nitric oxide measurement using electrochemical methods. Methods Enzymol 268 58-69... 

Ichimori K, Ishida H, Fukahori M, Nakazawa H, Murakami E (1994) Practical nitric oxide measurement tanploying a nitric oxide-selective electrode. Rev Sci lustrum 65 2714—2718... 

Santos RM, Rodrigues MS, Laranjinha J (2013) Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain. Biosens Bioelectron 44 152-159... 

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