Soybean nitrate reductase

In plant leaves, nitrate reduction requires NADH produced by glycolytic activity (13), The ozone-induced depression of glycolytic metabolism in soybean leaves was also reflected in a depressed rate of nitrate reduction ( ), A single ozone exposure depressed the vivo nitrate reductase (NR) activity about 60% (Table III), To determine if ozone affected the NR protein directly, the in vitro NR activity was determined in leaf extracts from plants exposed to 0 and 980 pg/m ozone. 

Callaci, J.J. Smarrelli, J., Jr. (1991). Regulation of the inducible nitrate reductase isoform from soybeans. Biochimica et Biophysica Acta 1008, 127-30. 

Streit, L., Martin, B.A. Harper, J.E. (1987). A method for the separation and purification of the three forms of nitrate reductase present in wild-type soybean leaves. Plant Physiology 84, 654-7. 

Genes affecting reaction to nutritional factors including phosphorus, iron, and chloride and the presence/absence of a constitutive nitrate reductase enzyme occur in soybean. Palmer et al. (2004) discuss these as well as leaf flavonol glucosides. 

Dean, J. V. and J. E. Harper, Nitric oxide and nitrous oxide production by soybean and winged bean during the in vivo nitrate reductase assay. Plant Physiol., 82, 718-723 (1986). 

Nitrate Reductase. Nitrate reductase is a name applied to certain flavoproteins containing molybdenum that reduce nitrate to nitrite at the expense of reduced pyridine nucleotides. The enzyme from Neuro-spora requires TPNH, one from soybean leaves uses either TPNH or DPNH, while a preparation from the bacteria of soybean root nodules uses only DPNH. These enzymes require molybdenum and FAD. 

---