Neutral lactase

Enzymes (lactase) 1,250 neutral lactase unity 4 drops 3-4 drops taken with milk or dairy product... 

Application and Principle This procedure is used to determine the neutral lactase activity of enzyme preparations derived from Kluyveromyces marxianus var. lactis and Saccharomyces sp. The assay is based on a 10-min hydrolysis of an o-nitrophenyl-fl-D-galactopyranosidc (ONPG) substrate at 30.0° 0.1° and at pH 6.50. 

Calculation for NLU Activity One Neutral Lactase Unit (NLU) is defined as that quantity of enzyme that will liberate 1.30 (imol/min of o-nitrophenol under the conditions of the assay. Calculate the activity of the enzyme preparation taken for the analysis as follows ... 

Neutral lactase (p-Galactosidase) is an enzyme produced on an industrial scale, which catalyzes the hydrolysis of lactose. Industry utilizes this enzyme in the production of low-lactose dairy products, whey treatment, and fermented lactic products. The goal of this study is to demonstrate that a new colorimetric method to measure the enzymatic activity is adequate for use as a standard method. 

In samples 1, 4, 6, and 10, the expected activity is 5000 NLU/g, whereas in the rest of the samples the expected activity is 2000 NLU/g. The data presented are averages of two replicates samples. The underlined data were later identified as outliers and were not used in further calculations. Intralaboratory (RSDr) and interlaboratory (RSDr) precision values are also shown. [Reprinted, with permission, from A. J. Engelen and P.H.G Randsdorp, Journal of AOAC International 82 No. 1, 1999, 112—118. Determination of Neutral Lactase Activity in Industrial Enzyme Preparations by a Colorimetric Enzymatic Method Collaborative Study . 1999 by Association of Official Analytical Chemists.]... 

Neutral-lactase. See p-Galactosidase Neutral potassium chromate. See Potassium... 

Lactozym 1500 L, type GP and 3000 L, type HP Neutral-lactase Yeast Lactase L-50,000 232-877-2 Aldomax GA-100 AMG 200 L Amyloglucosidase Diazyme L-200 Distillase L-200... 

Lactose may be hydrolysed to glucose and galactose by enzymes (fi-galactosidases, commonly called lactase) or by adds. Commercial sources of / -galactosidase are moulds (especially Aspergillus spp.), the enzymes from which have acid pH optima, and yeasts (Kluyveromyces spp.) which produce enzymes with neutral pH optima. /S-Galactosidases were considered to have... 

Lactase (Neutral) (P-Galactosidase) Activity, 801 Lipase Activity, 803 Lipase/Esterase (Forestomach) Activity, 804... 

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