Neurotensin/Neuromedin

Neurotensin/Neuromedin N. Table 1 Structures and and potential therapeutical uses of NT receptor ligands... 

Neurotensin/Neuromedin N. Table 2 NTS1 and NTS2 signaling properties... 

Dobner PR, Barber DL, Villa-Komaroff L, McKiernan C (1987) Cloning and sequence analysis of cDNA for the canine neurotensin/neuromedin N precursor. Proc Nat Acad Sci 34 3516-3520. 

Villeneuve P, Feliciangeli S, Croissandeau G, Seidah NG, Mbikay M, Kitabgi P, Beaudet A. Altered processing of the neurotensin/ neuromedin N precursor in PC2 knock down mice a biochemical 63. and immunohistochemical study. J. Neurochem. 2002 82 783-793. 

Tischler, AS. Ruzicka, A. Dobner, P.R. (1991). A pa-otein kinase inhibitor, staurosporine, mimics nerve growth factor induction of neurotensin/neuromedin N gene expression. J Biol Chem, Vol. 266(2), pp. 1141-6. 

Like all neuropeptides, NT is synthesized as part of a larger precursor that also contains neuromedin N (NN), a 6 amino acid neurotensin-like peptide (Table 1). Pro-NT/NN is processed in the regulated secretory pathway of neuroendocrine cells by prohormone convertases PCI, PC2 and PC5-A that belong to a larger family of proprotein convertases. Due to differential cleavage specificity and tissue distribution of the convertases, pro-NT/NN processing gives rise to approximately a 1 1 and a 5 1 ratio of NT over NN content in the brain and gut, respectively. The peptides are stored in secretory vesicles and released from neuroendocrine cells in a Ca2+-dependent manner. NT and NN actions are terminated by desensitization of the... 

Neurotensin (NT) is a tridecapeptide that was first isolated from the central nervous system but subsequently was found to be present in the gastrointestinal tract and in the circulation. It is synthesized as part of a larger precursor that also contains neuromedin N, a six-amino-acid NT-like peptide. 

In the brain, processing of the precursor leads primarily to the formation of neurotensin and neuromedin N these are released together from nerve endings. In the gut, processing leads mainly to the formation of neurotensin and a larger peptide that contains the neuromedin N sequence at the carboxyl terminal. Both peptides are secreted into the circulation after ingestion of food. 

Kislauskis E, Bullock BP, McNeil S, Dobner PR (1988) The rat gene encoding neurotensin and neuromedin N J Biol Chem 263 4963 1968. 

Nicot A, Bidard JN, Kitabgi P, Lhiaubet AM, Masuo Y, Palkovits M, Rostene W, Berod A (1995a) Neurotensin and neuromedin N brain levels after fornix transsection evidence for an efficient neurotensin precursor processing in subicular neurons. Brain Res 702 279-283. 

Carboxypeptidase is a unique 34.5 kDa metalloproteinase which removes the carboxyl terminal residues from a range of peptides, including angiotensin, leu -enkephalin, kinetensin, neuromedin N and neurotensin (Goldstein etal., 1989, 1991 Bunnett etal., 1992). As carboxypeptidase is released together with chymase, cathepsin G and proteoglycan in a 400-500 kDa complex, it is likely to act in concert with the other enzymes to degrade proteins. 

Neuromedin N (NN), H-Lys-Ile-Pro-Tyr-Ile-Leu-OH, a 6-peptide with a similar biological profile to neurotensin (NT) isolated from porcine spinal cord. It exhibited a contractile activity on guinea pig Ueum. NN is synthesized as part of a larger precursor which also contains neurotensin (NT) and neurotensin-like peptide. In the brain, pro-... 

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