Neuropeptides antisera

Shadiack AM, Sun Y, Zigmond RE (2001) Nerve growth factor antiserum induces axotomy-like changes in neuropeptide expression in intact sympathetic and sensory neurons. J Neurosci 27 363-371. 

Okada, K., Sugihara, H., Minami, S. Wakabayashi, I. (1993) Effect of parenteral administration of selected nutrients and central injection of gamma-globulin from antiserum to neuropeptide Y on growth hormone secretory pattern in food-deprived rats. Neuroendocrinology 57, 678-686. 

The objective of the present chapter is to provide a few standard protocols for immunocytochemical detection of neuropeptides, including double and triple labeling techniques that utilize difierent primary antisera/antibodies. It is very important to remember that ICC protocols have been modified in almost every laboratory that employs the technique, and that almost every antiserum or tissue requires specific modifications. Thus, it is wise to consult the original papers describing initial characterization of the antiserum and its application to specific tissues and animal species. For commercially available antisera, standard protocols are commonly supplied. The protocols presented here should provide a starting point for peptide ICC, and we suggest points at which modifications can be made. 

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