Neomycin phosphotransferase

Maximum residue limit Mass spectrometry Tandem mass spectrometry Material safety data sheet North American Free Trade Act N-Hydroxysuccinimide Nitrogen-phosphorus detection Neomycin phosphotransferase II Optical density Office of Plant Protection and Quarantine... 

Neomycin phosphotransferase II (NPTII) extraction from cotton leaves and cottonseed. The extraction buffer consists of 100 mM Tris, lOmM sodium borate, 5mM magnesium chloride, 0.2% ascorbate and 0.05% Tween 20 at pH 7.8. The frozen leaf sample is homogenized in cold (4 °C) buffer. An aliquot of the homogenate is transferred to a microfuge tube and centrifuged at 12 000 g for 15 min. The supernatant is diluted and assayed directly by ELISA. 

In mice, the bacterial neomycin phosphotransferase gene, which confers resistance to aminoglycoside antibiotics, prevented the loss of hair cells after neomycin treatment (142). 

Many other heterologous proteins have been expressed in tobacco chloroplast, and in many cases yields exceeding 5% of TSP were reported (Table 6.2). Examples of such proteins are neomycin phosphotransferase... 

---