Negative proteins

Chan, T.-W. D. Thomas, I. Colburn, A. W. Derrick, P. J. Initial velocities of positive and negative protein molecule-ions produced in matrix-assisted ultraviolet laser desorption using a liquid matrix. Chem. Phys. Lett. 1994,222,579-585. 

Inhibition of clathrin function can be achieved by different approaches (Table 1). Here, we describe the most commonly used inhibitory drugs and the newer and more specific molecular inhibitors in the form of dominant negative proteins. 

Health situations leading to positive and negative protein balance... 

Two independent kindreds with insulin-resistant type 2 diabetes in association with novel PPARy mutations were recently described (64). In both cases, the mutations—Val—>Met290 and Pro—>Leu467—were shown to function as dominant-negative proteins when expressed in transfected cells. This compelling story provides further proof of the role of PPARy as a potential regulator of in vivo insulin action. 

A phenomenon that is less well understood is that of false negatives, proteins that are known to interact but do not activate transcription in the Y2H system. While false negatives may not seem like much of a problem for molecular evolution experiments where the goal is just to evolve a protein with the desired property, it should be recalled that typically the assumption is made at intermediate rounds of selection that consensus sequences are emerging. There are many anecdotal reports of false negatives. The... 

Turbidimetric Titrations. The abrupt turbidity increases in Figure 1 correspond to phase separation of the polymer-protein complex. pHcritical increases with I because the attractive Coulombic interactions between the protein and polyion are screened by added salt, so that a larger net negative protein charge is required for phase separation (10). 

APPROACH TO AMINO ACID AND NEGATIVE PROTEIN BALANCE... 

Then, as a first step analysis, it can be proposed that CTX can adsorb onto the polymer surface where it occupies negatively charged sites. Assuming that the area occupied at such an interface is the same as at the lipid surface, i.e. 500 A, the area available for negative protein can be roughly estimated at about 2500 cm per mg of polymer. This area is compatible with the total covering of homogeneous spherical beads of about 0.02 pm mean diameter. 

Citrate ions at high concentrations appear to effectively compete with the negative protein for surface sites They show no evidence for competing with the positively charged protein ... 

Since the sequence is also Li ) Na ) K in the ion spectrum of negative proteins and these ions bring about fairly strong complex relations in this case also, the interpretation with regard to the cation sequence in the suppression of the tricomplex flocculation is exactly the same as that described in detail above. For the anion sequence, arguing similarly as above, the ion sequence in the ion spectrum of gelatin (p. 299, Fig. 22) is to be expected. This is indeed the same as that found experimentally in the suppression of the tricomplex. 

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