Nanoscale mass analyzers

In this study, K46 cells (5 x 10 ) were lysed in cell lysis buffer and immunoaffinity purified, as described above. Shown in Figure 1.3 is a gel separation of proteins from a representative preparation, suggesting the presence of many potentially complexed proteins. Bound proteins were eluted from the beads with citric acid and pH was neutralized with Tris. Following in-solution digestion, -30% of the sample was analyzed by nanoscale liquid chromatography-tandem mass spectrometry (nano-LC/... 

Bile acids, alcohols, and side-chain-shortened steroids have been extensively analyzed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) [1-3].The mobile phase usually features water/ methanol or water/acetonitrile containing an organic acid or buffer. As ESI is a concentration-dependent process, maximum sensitivity is obtained with high-concentration, low-volume samples run at low flow rate [26,27]. We have extensively used low-flow-rate LC-ESI-MS to achieve maximum sensitivity for steroid analysis [26], and with the advent of reliable nanoscale liquid chromatography (nano-LC) equipment, this form... 

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