NADH patents

Recent European patents,645,646 drawing on much earlier work647 649 describe an assay for NADH based on its ability to reduce naphthotri-azolium salts (236) to the azo dye (237) in close similarity to tetrazolium salts. 

The stabilized, orally absorbable form of NADH (ENADA) is a nutritional supplement available in the U.S. since 1995 and in the E.U. since 1997. Based on the patented formulation of this supplement, a number of clinical trials have been launched to prove scientifically that ENADA is effective. In order to get these studies started, an investigational new drag (IND) application was filed with the Food and Drag Administration (FDA). For FDA approval, it must be documented that ENADA (the stabilized oral form of NADH) is safe. For this reason, the maximum tolerated intravenous dose (MTD) of (3NADH (reduced form of beta-nicotinamide adenine... 

Birkmayer, J.G.D., Stable, ingestable and absorbable NADH and NADPH therapeutic compositions, U.S. Patent 5, 332, 727,1994. 

Another system using enzyme amplification has been patented by IQ Bio Ltd, Cambridge, UK. Alkaline phosphatase was used as a DNA label. The electron transfer connected with the reoxidation of NADH back to NAD" may be followed either photometrically or electrochem-ically [167]. 

Weianann C (1915) Improvements in bacterial fermentation of carbohydrates and in bacterial cultures for the same. British Patent 4845 Welch RW (1991) Purification and studies of two butanol (ethanol) dehydrogenases and the effects of rifampicin and chloramphenicol on other enzymes important in the production of butyrate and butanol in Clostridium acetobutylicum ATCC 824. PhD thesis. Rice University, Houston TX Welch RW, Rudolph FB, Papoutsakis ET (1989) Purification and characterization of the NADH-dependent butanol dehydrogenase from Clostridium acetobutylicum (ATCC 824). Arch Biochem Biophys 273 309-318... 

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