NADH/FMN oxidoreductase

An isolated protein having NADH FMN oxidoreductase activity having the amino acid sequence2 set forth in SEQ ID No 2 or an enzymatically active mutant thereof. 

A BDS method consisting in the incubation of a mixture formed by a fossil fuel and an aqueous phase containing a biocatalyst and a rate-enhancing amount of a protein having NADH FMN oxidoreductase activity or enzymatically active mutant thereof. The oxidoreductase has the amino acid sequence set forth in SEQ ID No. 2, described in the original document (see Ref. [57]). A separation stage is also claimed. 

Hydroxy-steroid dehydrogenases co-immobilized on Sepharose 4B with NADH FMN oxidoreductase and luciferase provide light-emission assays for picomole levels of androsterone or testosterone.145... 

Bioluminescence bioassays based on luminous bacteria and coupled enzyme system NADH-FMN-oxidoreductase-luciferase were adapted for monitoring the saline-water conditions of Lake Shira (Khakasia, Siberia). The differences in bioluminescence responses have been found to be related to the salt composition and the oxidation-reduction properties of water. Bioluminescent kinetics parameters, which are mostly sensitive to pollution under conditions of saline water, have been observed. Figure 1 shows the typical bioluminescence kinetics of the samples of water due to anthropogenic influence (beach) and control clear water (non-recreational area). 

The reactions leading to light emission involve a fatty acid reductase complex (reaction [II]), NADH FMN oxidoreductase (EC 1.6.99.3) or NADPH EMN oxidoreductase (EC 1.6.99.1) (reaction [III]), and bacterial luciferase (EC 1.14.14.3) (reaction [IV]). The fCn, values for NADH and NADPH with the oxidoreductases of the appropriate selectivity are 23 and 22 gmol 1 respectively. Eor luciferase the fCn, for EMNH2 is 0.3 pmoll . In the luciferase reaction enzyme-bound FMNH2 is oxidized to a 4a-peroxy flavin intermediate and this reacts with tet-radecanal to form a stable intermediate that decays slowly with the emission of light. The aldehyde (tet-radecanal) is oxidized to tetradecanoic acid ... 

Immunoassay and nucleic acid probe assays Labels. recApoaequorin, firefly luciferase, marine bacterial luciferase, Vargula luciferase Detection reactions, alkaline phosphatase label (luciferin-O-phosphate/firefly luciferase), glucose 6-phosphate dehydrogenase label (marine bacterial luciferase/NADH FMN oxidoreductase reaction)... 

Reduction of the 5-deaza-analogue of FMN under a catalysis of NADH /FMN oxidoreductase was investigated (Fisher and Walsh 1974) and direct transfer of the pro-R hydrogen of NADH onto the 5 position of 5-deazaflavin was observed (Scheme 28). 

DszD SnaC—NADH FMN oxidoreductase Streptomyces—1711412) 37% (51%) NADH/FMN... 

One example is glutamate dehydrogenase (GDH), which is immobilized on the tip of an optical fiber in the presence of NADH-FMN oxidoreductase and luciferase. The GDH catalyses successively the following reactions [206] ... 

Bacterial luciferase can be used in the assay of NAD(P)H by a coupling technique. In the first step flavin mononucleotide (FMN) is reduced by NAD (P)H and NADH/FMN-oxidoreductase. FMNH2 then reacts with oxygen, a long chain aldehyde, and bacterial luciferase to give FMN, carboxylic acid, water and light (as described on p. 156) [111]. 

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