NAD P H-Alcohol Dehydrogenase Substrates

We have also used p-rosaniline as an alternative to the tetrazolium salts. This 

Another alternative is the use of oxygenases, which oxidize the reduced coenzymes and simultaneously incorporate molecular oxygen into the other substrate that is converted into a dye. The oxygenase reaction can be carried out in such a way that HjOj is produced and determined by a variety of methods. A third method to produce color with NAD(P)H consuming enzymes is the reduction of nitroso compounds by ADH the substrate is oxidized, the p-nitrosodime-thylaniline is reduced, and the cofactor is recycled between both reactions [58]. 

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