Dehydrogenases substrate

N. Mukerjee, R. Peitruszko, Human Mitochondrial Aldehyde Dehydrogenase Substrate Specificity-Comparison of Esterase with Dehydrogenase Reaction , Arch. Biochem. Biophys. 1992, 299, 23-29. 

K. Vogel, K. L. Platt, P. Petrovic, A. Seidel, F. Oesch, Dihydrodiol Dehydrogenase Substrate Specificity, Inducibility and Tissue Distribution , Arch. Toxicol. 1982, Suppl. 5, 360 - 364. 

Fig. 30. Horse liver alcohol dehydrogenase substrate binding pocket, unoccupied. Stereo drawing from the work of Branden and colleagues. 

Scheme 1.1 Chiral fluorogenic alcohol dehydrogenase substrates and assay conditions.

K. Palczewski, S. JSger, J. Buczylko, R. Crouch, D. L. Bredberg, K. P. Hofmann, M. A. Asson-Batres, and J. C. Saari. Rod outer segment retinol dehydrogenase substrate specificity and role in phototransduction. Biochemistry, 33, 13741-13750, 1994. 

I. Morgunov and P.A. Srere. 1998. Interaction between citrate synthase and malate dehydrogenase Substrate channeling of oxaloacetate J. Biol. Chem. 273 29540-29544. (PubMed)... 

Ramaswamy, S., M. el Ahmad, O. Danielsson, H. Jornvall and H. Eklund. Crystal structure of cod liver class I alcohol dehydrogenase substrate pocket and structurally variable segments. Protein Sci. 5 663-671, 1996. 

Fig. 55. Schematic representation of a reagentless enzyme electrode using electrochemical regeneration of NAD for the determination of reduced dehydrogenase substrates.

Kim MJ, Whitesides GM (1988) L-lactate dehydrogenase substrate spedflcity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids. J Am Chem Soc 110 2959-2962... 

Fig. 16 (A) Ru(bpy)3 based ECL reaction mechanism for detection NAD+-dehydrogenase substrates and (B) Ru(bpy)3 based ECL reaction ECL reaction mechanism for detection of oxidase substrates through ECL Inhibition. Reprinted with permission from Ref 17. Copyright (2011) Elsevier.

Besides flavin oxidoreductases the appropriate dehydrogenase systems could also be used for assaying sugars and other dehydrogenase substrates (e.g., ethanol, lactate, and amino acids). In contrast to FAD,... 

Palczewski K, Jager S, Buczylko J, Crouch RK, Bredberg DL, Hofmaim KP, Asson-Batres MA, Saari JC (1994) Rod outer segment retinol dehydrogenase Substrate specificity and role in phototransduction. Biochemistry 33 13 741-13 750... 

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