N-hydroxysucdnimide ester

YAN 94] Yan M., Cai S.X., Wybourne M.N. et al., N-Hydroxysucdnimide Ester Functionalized Perfluorophenyl Azides as Novel Photoactive Heterobifunctional Crosslinking Reagents. The Covalent Inunobilization of Biomolecules to Polymer Smfaces , Bioconjugate Chemistry, vol. 5, pp. 151-157, 1994. 

Synthesis of triazine haptens and hapten-protein conjugates. Simazine and atrazine were derivatized with mercaptopropionic add (mpa) at Rl, or aminohexanoic acid (aha) at R2, and these haptens were covalently linked to keyhole limpet hemoyanin (KLH), conalbumin (CON), or bovine serum albumin (BSA), by forming active esters with N-hydroxysucdnimide (g) (Figure 1, structures III and IV). This technique was also used to couple simazine-aminohexanoic add to calf intestine alkaline phosphatase (Figure 1, structure V), for use as the %aptenated enzyme in the EIA format described below. 

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