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Crosslinking reagents heterobifunctional

Carlsson, J., Drevin, H., and Axen, R. (1978) Protein thiolation and reversible protein-protein conjugation. N-succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional crosslinking reagent. Biochem. J. 173, 723—737. [Pg.143]

YAN 94] Yan M., Cai S.X., Wybourne M.N. et al., N-Hydroxysucdnimide Ester Functionalized Perfluorophenyl Azides as Novel Photoactive Heterobifunctional Crosslinking Reagents. The Covalent Inunobilization of Biomolecules to Polymer Smfaces , Bioconjugate Chemistry, vol. 5, pp. 151-157, 1994. [Pg.326]

The greatest degree of control in crosslinking procedures is afforded using heterobifunctional reagents (Chapter 5). Since a heterobifunctional crosslinker has different reactive groups on either end of the molecule, each side can be directed specifically toward different functional... [Pg.33]

Sulfosuccinimidyl-2-(7-azido-4-methylcoumarin-3-acetamide)ethyl-l,3 -dithiopropionate (SAED) is a photoreactive heterobifunctional crosslinking agent that also contains a fluorescent group (Thermo Fisher). The sulfo-NHS ester end of the reagent reacts with primary amines in proteins and other molecules to form stable amide linkages. The photoreactive end is an AMCA... [Pg.316]

Sulfo-SAMCA, sulfosuccinimidyl-7-azido-4-methylcoumarin-3-acetate, is a heterobifunctional reagent similar in design to SAED (Section 3.9, this chapter) (Thermo Fisher). One end of the crosslinker contains an amine-reactive sulfo-NHS ester, while the other end is an AMCA derivative (Chapter 9, Section 3) that contains a photosensitive phenyl azide group. Unlike... [Pg.319]

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See also in sourсe #XX -- [ Pg.276 ]



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