Myrothecium antibiotics

An example of a separation primarily based on polar interactions using silica gel as the stationary phase is shown in figure 10. The macro-cyclic tricothecane derivatives are secondary metabolites of the soil fungi Myrothecium Verrucaia. They exhibit antibiotic, antifungal and cytostatic activity and, consequently, their analysis is of interest to the pharmaceutical industry. The column used was 25 cm long, 4.6 mm in diameter and packed with silica gel particles 5 p in diameter which should give approximately 25,000 theoretical plates if operated at the optimum velocity. The flow rate was 1.5 ml/min, and as the retention time of the last peak was about 40 minutes, the retention volume of the last peak would be about 60 ml. 

Haerri, E., Loeffler, W., Sigg, H. P., Staehelin, H., Stoll, C., Tamm, C., and Wiesinger, D. (1962). On the verrucarins and roiidins, a group of highly cytostatically active antibiotics from Myrothecium species. Helv. Chim. Acta 45, 839-853. 

Okuchi M, Itoh M, Kaneko Y, Doi S (1968) A New Antifungal Substance Produced by Myrothecium. Agric Biol Chem 32 394 idem (1973) Sesquiterpene Antibiotic, Compound A-2, Minor Component from Myrothecium, I. Fermentation, Isolation, Physicochemical Properties, and the Stracture. J Antibiot 26 562... 

Jarvis BB, Vrudhula VM (1983) New Trichoverroids from Myrothecium verrucaria 16-Hydroxytrichodermadienediols. J Antibiot 36 459... 

Bohner B, Fetz E, Harri E, Sigg HP, Stoll Ch, Tamm Ch (1965) Verrucarine und Roridine, 8. Mitteilung. Uber die Isolierung von Verrucarin H, Verrucarin J, Roridin D und Roridin E aus Myrothecium-Aiten. Helv Chim Acta 48 1079 Smitka TA, Bunge RH, Bloem RJ, French JC (1984) Two New Trichothecenes, PD 113325 and PD 113326. J Antibiot 37 823... 

Jarvis BB, Stahly GP, Pavanasasivam G, Mazzola EP (1980) Stnicture of Roridin J, a New Macrocyclic Trichothecene from Myrothecium verrucaria. J Antibiot 33 256... 

The chief metabohte of most strains of Myrothecium verrucaria is the antibiotic verrucarin A, C27H34O9, for which structure I has been established by x-ray analysis (McPhail and Sim, 1965) and degradation (Gutzwiller and Tamm, 1965). Muconomycin A, isolated from the same source (Vittimberga, I963), appears to be identical to verrucarin A (Godtfredsen and Vangedal, 1965)-Verrucarin A (I), which is accompanied by related substances termed verru-carins and roridins, is the most abundant of these substances in M. verrucaria S 118, S 750 and S 833 it is found in M. roridum S 1135 but not in S 973 (Harri et al, 1962). 

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