Mutants distribution patterns

Figure 5. (A) Proposed mechanism for the formation of aromatic polyketides by OKS and its mutants. The enzymes catalyze chain initiation/elongation, and possibly initiating the first aromatic ring formation reaction at the methyl end of the polyketide intermediate. The partially cyclized intermediate are then releasedfrom the active-site and undergo spontaneous cyclizations, which leads to formation of the fused ring systems. (B) Distribution pattern of aromatic polyketides produced by OKS and its mutants. (Reproduced from reference 7b. Copyright 2005 American Chemical Society.)...

The results in Table I show that, in the presence of plant material, the standard GA3 is spread over a considerably wider range than is usual. However, after the sample is further purified by the second run, the mobility of GA3 returns to normal. The same pattern of distribution, with zone III as maximum, is manifest in the natural run, although at considerably lower fluorescent intensities. For both fractions, the presence of GA3 is confirmed by the capryl system. A portion of zone III of the natural extract gave positive response proportional to concentration in the pinto bean seedling assay and in the dwarf maize mutants I and V assay. The relative activity on both mutants was approximately equal, as is required for GA3 (26). The correlation of relatively specific biological growth activity with chromatographic and chemical behavior affirms the presence of a GA3-like substance in kudzu vine. 

FIGURE 2. Comparison of Anacystis wildtype (PCC 7942) with herbicide tolerant mutants concerning the rate of synthesis of the D1 protein. Distribution of radioactivity in the polypeptide pattern of whole cells after 18 min. exposure to 35 methionine. 

Cytoehrome function is inseparable from that of mitochondria, and the inheritance of the cytochrome patterns of the petite and poky (see below) mutants has long been known to follow the 4 0 distribution of maternal or cytoplasmic inheritance, as opposed to the 2 2 distribution of chromosomal genes. That the biochemical machinery for cytoplasmic inheritance exists has been shown by the finding of the necessary genetic and protein-synthesizing components in the mitochondrion (for references, see [42,45,46]). It appears likely that the amount of intrinsically synthesized protein, although indispensable, is small compared to extrinsically produced material. 

It is interesting to note that the O mutant sites are not randomly distributed throughout the C gene, but are concentrated in the left half of the gene. There does not seem to be any pattern, as yet discernible,... 

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